Overview

  • Product name

    Anti-Fc epsilon RI/FCER1A antibody [CRA1] (Biotin)
    See all Fc epsilon RI/FCER1A primary antibodies
  • Description

    Mouse monoclonal [CRA1] to Fc epsilon RI/FCER1A (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Fc epsilon RI/FCER1A aa 1-84.
    Sequence:

    MAPAMESPTL LCVALLFFAP DGVLAVPQKP KVSLNPPWNR IFKGENVTLT CNGNNFFEVS STKWFHNGSL SEETNSSLNI VNAK

  • Epitope

    corresponds to Amino acids 1-84 of Human Fc epsilon RI/FCER1A
  • Positive control

    • CHO/alpha beta gamma cells
  • General notes

    [biotin]/[IgG] = 8.9

     This product was previously labelled as Fc epsilon RI

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. Filter sterilized.
  • Clonality

    Monoclonal
  • Clone number

    CRA1
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
Flow Cyt Use at an assay dependent concentration.

ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • Sequence similarities

    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fc epsilon RI alpha antibody
    • Fc epsilon RI alpha chain antibody
    • Fc epsilon RI alpha-chain antibody
    • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
    • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
    • Fc IgE receptor alpha polypeptide antibody
    • Fc IgE receptor, alpha chain antibody
    • Fc IgE receptor, alpha polypeptide antibody
    • Fc of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc-epsilon RI-alpha antibody
    • FCE 1A antibody
    • FCE1A antibody
    • FCER 1A antibody
    • Fcer1a antibody
    • FCERA_HUMAN antibody
    • FceRI alpha antibody
    • FcERI antibody
    • high affinity IgE receptor antibody
    • High affinity immunoglobulin epsilon receptor alpha subunit antibody
    • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
    • High affinity immunoglobulin epsilon receptor subunit alpha antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor subunit alpha antibody
    • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
    • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
    see all

Images

  • FACS analysis of CHO/alpha beta gamma cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining, using FITC-labeled secondary antibody.

References

This product has been referenced in:

  • Untersmayr E  et al. The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. PLoS One 5:e9023 (2010). Read more (PubMed: 20126404) »
  • Takai T  et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). Read more (PubMed: 11272849) »
See all 4 Publications for this product

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