Key features and details
- Mouse monoclonal [CRA2] to Fc epsilon RI/FCER1A (Biotin)
- Suitable for: WB, ICC, Flow Cyt, Inhibition Assay
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-Fc epsilon RI/FCER1A antibody [CRA2] (Biotin)
See all Fc epsilon RI/FCER1A primary antibodies
DescriptionMouse monoclonal [CRA2] to Fc epsilon RI/FCER1A (Biotin)
Tested applicationsSuitable for: WB, ICC, Flow Cyt, Inhibition Assaymore details
Species reactivityReacts with: Human
- CHO/alpha beta gamma cells
[biotin]/[IgG] = 6.9
This product was previously labelled as Fc epsilon RI
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityIon Exchange Chromatography
Purification notesThe IgG fraction was purified from serum free culture medium of mouse hybridoma by propriety chromatography under mild conditions. Filter sterilized.
Light chain typekappa
Our Abpromise guarantee covers the use of ab122915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Inhibition Assay||Use at an assay dependent concentration. Inhibition of binding of IgE with Fc epsilon RI.|
FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- Fc epsilon RI alpha antibody
- Fc epsilon RI alpha chain antibody
- Fc epsilon RI alpha-chain antibody
ab122915 has been referenced in 5 publications.
- Takai T et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). PubMed: 11272849
- Goto T et al. Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis. J Dermatol Sci 27:156-61 (2001). PubMed: 11641054
- Takai T et al. Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci Biotechnol Biochem 64:1856-67 (2000). PubMed: 11055388
- Hasegawa S et al. Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes. Blood 93:2543-51 (1999). PubMed: 10194433
- Hakimi J et al. The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J Biol Chem 265:22079-81 (1990). PubMed: 2148316