Anti-Fc epsilon RI/FCER1A antibody [CRA2] (Biotin) (ab122915)

Overview

  • Product name

    Anti-Fc epsilon RI/FCER1A antibody [CRA2] (Biotin)
    See all Fc epsilon RI/FCER1A primary antibodies
  • Description

    Mouse monoclonal [CRA2] to Fc epsilon RI/FCER1A (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, ICC, Flow Cyt, Inhibition Assaymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Fc epsilon RI/FCER1A aa 85-172.
    Sequence:

    FEDSGE YKCQHQQVNE SEPVYLEVFS DWLLLQASAE VVMEGQPLFL RCHGWRNWDV YKVIYYKDGE ALKYWYENHN ISITNATVED SG

  • Positive control

    • CHO/alpha beta gamma cells
  • General notes

    [biotin]/[IgG] = 6.9

     This product was previously labelled as Fc epsilon RI

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    The IgG fraction was purified from serum free culture medium of mouse hybridoma by propriety chromatography under mild conditions. Filter sterilized.
  • Clonality

    Monoclonal
  • Clone number

    CRA2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Inhibition Assay Use at an assay dependent concentration. Inhibition of binding of IgE with Fc epsilon RI.

Target

  • Function

    Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
  • Sequence similarities

    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fc epsilon RI alpha antibody
    • Fc epsilon RI alpha chain antibody
    • Fc epsilon RI alpha-chain antibody
    • Fc fragment of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc fragment of IgE, high affinity I, receptor for, alpha subunit antibody
    • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide antibody
    • Fc IgE receptor alpha polypeptide antibody
    • Fc IgE receptor, alpha chain antibody
    • Fc IgE receptor, alpha polypeptide antibody
    • Fc of IgE high affinity I receptor for alpha polypeptide antibody
    • Fc-epsilon RI-alpha antibody
    • FCE 1A antibody
    • FCE1A antibody
    • FCER 1A antibody
    • Fcer1a antibody
    • FCERA_HUMAN antibody
    • FceRI alpha antibody
    • FcERI antibody
    • high affinity IgE receptor antibody
    • High affinity immunoglobulin epsilon receptor alpha subunit antibody
    • high affinity immunoglobulin epsilon receptor alpha-subunit antibody
    • High affinity immunoglobulin epsilon receptor subunit alpha antibody
    • IgE Fc receptor alpha subunit antibody
    • IgE Fc receptor subunit alpha antibody
    • Immunoglobulin E receptor high affinity of mast cells alpha polypeptide antibody
    • immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody
    see all

References

This product has been referenced in:

  • Takai T  et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). Read more (PubMed: 11272849) »
  • Goto T  et al. Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis. J Dermatol Sci 27:156-61 (2001). Read more (PubMed: 11641054) »
See all 5 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for your recent telephone enquiry.

As discussed on the telephone, I am sorry to confirm that as far as we are aware, ab93899 and ab122915have never been tested with samples from mouse. All tested species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

With regards to ab92899 PDGFC antibody, I have checked the alignment of the mouse protein with the immunogen. This is fairly high at 77%. It is therefore fairly likely the antibody could crossreact with mouse, though we are not able to guarantee this without further testing.

Regarding ab122915 Fc epsilon RI antibody [CRA2], this has only 50 -60 % alignment with the mouse protein. It has also been tested unsuccessfully in mouse so regrettably we would not recommend to try this on mouse samples.We do have the following Fc epsilon RI antibody which is tested in mouse which I hope may be of interest to you:

ab34362 Fc epsilon RI antibody
Tested in: IP, WB
Rabbit polyclonal
Reacts with: Hu, Ms, Rat
https://www.abcam.com/index.html?datasheet=34362 (or use the following: https://www.abcam.com/index.html?datasheet=34362).

In this case, I can offer our testing discount program if you would like to test ab92899 PDGFC antibody in mouse. We can providediscount off a future purchase if you buy ab92899 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab92899 in mouse. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchase ab92899 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab92899 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offerand I would be happy to help you further.

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