FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)

Overview

  • Product name
    FCCP, mitochondrial oxidative phosphorylation uncoupler
  • Description
    Potent mitochondrial oxidative phosphorylation uncoupler
  • Biological description
    Potent mitochondrial oxidative phosphorylation uncoupler (IC50 = 20 nM). Disrupts ATP synthesis by transporting protons across mitochondrial inner membranes. Depolarises mitochondrial membrane potential.
  • Purity
    > 99%
  • CAS Number
    370-86-5
  • Chemical structure
    Chemical Structure

Properties

Images

  • HT29 cells were treated with the indicated compounds ((etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) for 24 hours before analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XF96 extracellular flux analyzer. Baseline rates (black) were determined at the indicated times before the addition of oligomycin (green) and then FCCP (red). Rate data are normalized to per-well cell number determined by post-analysis high-content imaging.

References

This product has been referenced in:
  • Fossati G  et al. The mitochondrial network of human neutrophils: role in chemotaxis, phagocytosis, respiratory burst activation, and commitment to apoptosis. J Immunol 170:1964-72 (2003). Read more (PubMed: 12574365) »
  • Collins TJ  et al. Inositol 1,4,5-trisphosphate-induced Ca2+ release is inhibited by mitochondrial depolarization. Biochem J 347:593-600 (2000). Read more (PubMed: 10749691) »
  • Gautier I  et al. A moderate but not total decrease of mitochondrial membrane potential triggers apoptosis in neuron-like cells. Neuroreport 11:2953-6 (2000). Read more (PubMed: 11006972) »
  • Benz R & McLaughlin S The molecular mechanism of action of the proton ionophore FCCP (carbonylcyanide p-trifluoromethoxyphenylhydrazone). Biophys J 41:381-98 (1983). Read more (PubMed: 6838976) »
See all 13 Publications for this product

Customer reviews and Q&As

Answer



The concentration of CCCP or FCCP required as a positive control will vary based on cell line. Perhaps it would be useful to try 50uM FCCP as this has worked for you in the past.

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