Question (69683) | FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)

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Question

I chose 10uM FCCP because in your protocol given it says to use between 2-10UM of CCCP or FCCP. I decided to try 10uM because I have previously done JC-1 experiments with an Invitrogen kit and they recommended a concentration of 50uM so I just chose the highest concentration you suggested...

Answer



The concentration of CCCP or FCCP required as a positive control will vary based on cell line. Perhaps it would be useful to try 50uM FCCP as this has worked for you in the past.

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