Overview

  • Product name
    Anti-FCGBP antibody
    See all FCGBP primary antibodies
  • Description
    Rabbit polyclonal to FCGBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 289-417 of Human FCGBP.

  • Positive control
    • U 2 OS cells; Human rectum tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in the maintenance of the mucosal structure as a gel-like component of the mucosa.
  • Tissue specificity
    Mainly expressed in placenta and colon epithelium. Expressed in thyroid, and down-regulated in thyroid carcinomas. Present in serum, with higher levels in patients with various autoimmune diseases (at protein level).
  • Sequence similarities
    Contains 12 TIL (trypsin inhibitory-like) domains.
    Contains 13 VWFD domains.
  • Domain
    The N-terminal IgGFc-binding region is primate-specific.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Fc fragment of IgG binding protein antibody
      • FC(GAMMA)BP antibody
      • Fcgamma-binding protein antigen antibody
      • FcgammaBP antibody
      • FCGBP antibody
      • FCGBP_HUMAN antibody
      • Human Fc gamma BP antibody
      • IgGFc-binding protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGBP antibody (ab121202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGBP antibody (ab121202)
      ab121202 at 1/200 dilution staining FCGBP in Paraffin-embedded Human rectum tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-FCGBP antibody (ab121202)
      Immunocytochemistry/ Immunofluorescence - Anti-FCGBP antibody (ab121202)
      ab121202 at 4 µg/ml staining FCGBP in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab121202? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-FCGBP antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioma cell line)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Specification
    Glioma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Mrs. Vidya Nimbalkar

    Verified customer

    Submitted Apr 29 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up