Anti-FCGBP antibody (ab121202)

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Overview

  • Product name

    Anti-FCGBP antibody
    See all FCGBP primary antibodies

  • Description

    Rabbit polyclonal to FCGBP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 289-417 of Human FCGBP.

  • Positive control

    • U 2 OS cells; Human rectum tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in the maintenance of the mucosal structure as a gel-like component of the mucosa.

  • Tissue specificity

    Mainly expressed in placenta and colon epithelium. Expressed in thyroid, and down-regulated in thyroid carcinomas. Present in serum, with higher levels in patients with various autoimmune diseases (at protein level).

  • Sequence similarities

    Contains 12 TIL (trypsin inhibitory-like) domains.
    Contains 13 VWFD domains.

  • Domain

    The N-terminal IgGFc-binding region is primate-specific.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Fc fragment of IgG binding protein antibody
      • FC(GAMMA)BP antibody
      • Fcgamma-binding protein antigen antibody
      • FcgammaBP antibody
      • FCGBP antibody
      • FCGBP_HUMAN antibody
      • Human Fc gamma BP antibody
      • IgGFc-binding protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGBP antibody (ab121202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGBP antibody (ab121202)
      ab121202 at 1/200 dilution staining FCGBP in Paraffin-embedded Human rectum tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-FCGBP antibody (ab121202)
      Immunocytochemistry/ Immunofluorescence - Anti-FCGBP antibody (ab121202)
      ab121202 at 4 µg/ml staining FCGBP in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab121202? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-FCGBP antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioma cell line)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Specification
    Glioma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Vidya Nimbalkar

    Verified customer

    Submitted Apr 29 2017

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