Recombinant
RabMAb

Recombinant Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

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Overview

  • Product name

    Anti-FCGRT/FCRN antibody [EPR22627-25]
    See all FCGRT/FCRN primary antibodies

  • Description

    Rabbit monoclonal [EPR22627-25] to FCGRT/FCRN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human FCGRT/FCRN aa 1-350. The exact sequence is proprietary.
    Database link: P55899

  • Positive control

    • WB: HEK-293 and THP-1 whole cell lysates; Human placenta tissue. IHC-P: Human spleen and liver tissue. Flow Cyt: THP-1 cells. IP: HEK-293 whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.
IP 1/30.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Alpha chain antibody
        • Fc fragment of IgG, receptor transporter, alpha antibody
        • FCGRN_HUMAN antibody
        • FCGRT antibody
        • FcRn alpha chain antibody
        • FcRn antibody
        • FCRN, alpha chain antibody
        • IgG Fc fragment receptor transporter alpha chain antibody
        • IgG Gc receptor antibody
        • IgG receptor FcRn large subunit p51 antibody
        • IgG receptor FcRn large subunit p51 precursor antibody
        • Immunoglobulin receptor, intestinal, heavy chain antibody
        • Neonatal Fc receptor antibody
        see all

      Images

      • Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution

        Lane 1 : Human placenta tissue lysate
        Lane 2 : Human placenta treated with PNGase F tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

        Predicted band size: 40 kDa


        Exposure time: 3 minutes


        Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

        Human FCGRT/FCRN possesses one potential glycosylation site. The molecular weight of deglycosylated FCGRT/FCRN is consistent with the literature (PMID: 12163559).

      • Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Western blot - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution

        Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
        Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
        Lane 3 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate
        Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 40 kDa



        Blocking/Diluting buffer and concentration: 5% NFDM/TBST. 

        Exposure times: Lane 1: 37 secs; Lane 2-4: 3 mins.

        Negative control: LNCaP (PMID: 27974681) and HeLa (PMID: 21216798).

      • Immunoprecipitation - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Immunoprecipitation - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

        FCGRT/FCRN was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab228975 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab228975 1/1000 dilution (0.55 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

        Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10μg
        Lane 2: ab228975  IP in HEK-293 whole cell lysate
        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228975 in HEK-293 whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 100 seconds.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

        Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID: 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

        Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen, arrows indicate the macrophages (PMID: 28402755) is observed .The section was incubated with ab228975 for 30 mins at room temperature.

        The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

        Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution (0.14 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human spleen (PMID: 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      • Flow Cytometry - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)
        Flow Cytometry - Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

        Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labeling FCGRT/FCRN with ab228975 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Bright fluorophore (like AF647) conjugated secondary antibodies are recommended to get good separation.

        Negative control: HeLa (PMID: 21216798).

        Gated on viable cells.

      References

      ab228975 has not yet been referenced specifically in any publications.

      Publishing research using ab228975? Please let us know so that we can cite the reference in this datasheet.

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