Recombinant
RabMAb

Recombinant Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975)

Overview

  • Product name

    Anti-FCGRT/FCRN antibody [EPR22627-25]
    See all FCGRT/FCRN primary antibodies
  • Description

    Rabbit monoclonal [EPR22627-25] to FCGRT/FCRN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FCGRT/FCRN aa 1-350. The exact sequence is proprietary.
    Database link: P55899

  • Positive control

    • WB: HEK-293 and THP-1 whole cell lysates; Human placenta tissue. IHC-P: Human spleen and liver tissue. Flow Cyt: THP-1 cells. IP: HEK-293 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.
IP 1/30.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha chain antibody
      • Fc fragment of IgG, receptor transporter, alpha antibody
      • FCGRN_HUMAN antibody
      • FCGRT antibody
      • FcRn alpha chain antibody
      • FcRn antibody
      • FCRN, alpha chain antibody
      • IgG Fc fragment receptor transporter alpha chain antibody
      • IgG Gc receptor antibody
      • IgG receptor FcRn large subunit p51 antibody
      • IgG receptor FcRn large subunit p51 precursor antibody
      • Immunoglobulin receptor, intestinal, heavy chain antibody
      • Neonatal Fc receptor antibody
      see all

    Images

    • All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human placenta treated with PNGase F tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 40 kDa


      Exposure time: 3 minutes


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

      Human FCGRT/FCRN possesses one potential glycosylation site. The molecular weight of deglycosylated FCGRT/FCRN is consistent with the literature (PMID: 12163559).

    • All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution

      Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
      Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
      Lane 3 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate
      Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 40 kDa



      Blocking/Diluting buffer and concentration: 5% NFDM/TBST. 

      Exposure times: Lane 1: 37 secs; Lane 2-4: 3 mins.

      Negative control: LNCaP (PMID: 27974681) and HeLa (PMID: 21216798).

    • FCGRT/FCRN was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab228975 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab228975 1/1000 dilution (0.55 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

      Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10μg
      Lane 2: ab228975  IP in HEK-293 whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228975 in HEK-293 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 100 seconds.

    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID: 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen, arrows indicate the macrophages (PMID: 28402755) is observed .The section was incubated with ab228975 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution (0.14 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human spleen (PMID: 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labeling FCGRT/FCRN with ab228975 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Bright fluorophore (like AF647) conjugated secondary antibodies are recommended to get good separation.

      Negative control: HeLa (PMID: 21216798).

      Gated on viable cells.

    References

    ab228975 has not yet been referenced specifically in any publications.

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