Key features and details
- Rabbit polyclonal to FCGRT/FCRN - Extracellular domain
- Suitable for: WB, ICC/IF, ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FCGRT/FCRN antibody - Extracellular domain
See all FCGRT/FCRN primary antibodies
DescriptionRabbit polyclonal to FCGRT/FCRN - Extracellular domain
Tested applicationsSuitable for: WB, ICC/IF, ELISA, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey
Recombinant fragment corresponding to Human FCGRT/FCRN aa 24-297 (extracellular).
AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGA WVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGP DNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANK ELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSA FSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHY CCIVQHAGLAQPLRVELESPAKSS
Database link: P55899
- WB: HepG2 and A549 whole cell lysates, mouse liver, kidney and spleen tissue lysates. IHC-P: Human placenta and human pancreatic cancer tissues. ICC/IF: HepG2 cells.
This product was previously labelled as FCGRT
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab193148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|ICC/IF||1/50 - 1/200.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/20 - 1/200.|
FunctionBinds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Cellular localizationCell membrane.
- Information by UniProt
- Alpha chain antibody
- Fc fragment of IgG, receptor transporter, alpha antibody
- FCGRN_HUMAN antibody
All lanes : Anti-FCGRT/FCRN antibody - Extracellular domain (ab193148) at 2 µg
Lane 1 : HepG2 whole cell lysate
Lane 2 : Mouse liver tissue
Lane 3 : Mouse spleen tissue
Lane 4 : A549 whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
All lanes : Anti-FCGRT/FCRN antibody - Extracellular domain (ab193148) at 5.3 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
All lanes : Polyclonal goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling FCGRT/FCRN with ab193148 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic cancer tissue labelling FCGRT/FCRN with ab193148 at a dilution of 1/100.
Immunocytochemistry/Immunofluorscence analysis of HepG2 cells labelling FCGRT/FCRN with ab193148 at a dilution of 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody.