Product nameAnti-FCHO1 antibody
See all FCHO1 primary antibodies
DescriptionRabbit polyclonal to FCHO1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Zebrafish
- 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
RelevanceFCHO1 is involved in clathrin-coated vesicle formation.
- FCH domain only 1 antibody
- FCH domain only protein 1 antibody
- FCHO 1 antibody
- KIAA0290 antibody
Anti-FCHO1 antibody (ab84740) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab84740 staining FCHO1 in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab84740 staining FCHO1 in human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
This product has been referenced in:
- Umasankar PK et al. A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing. Elife 3:N/A (2014). Read more (PubMed: 25303365) »
- Umasankar PK et al. Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning. Nat Cell Biol 14:488-501 (2012). Read more (PubMed: 22484487) »