Overview

  • Product name

    Anti-FCHSD1 antibody
  • Description

    Mouse polyclonal to FCHSD1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length human FCHSD1 protein (Isoform 3; AAH47016.1, 1 a.a. ~ 409 a.a)

  • Positive control

    • FCHSD1 transfected 293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: Isoform 3 46 kDa: Isoform 1 77kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-FCHSD1 antibody (ab67017) at 1/500 dilution

      Lane 1 : FCHSD1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 46 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Cao H  et al. FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro. PLoS One 8:e56516 (2013). Mouse . Read more (PubMed: 23437151) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. The estimated delivery date is xxx.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Answer

    DISCOUNT CODE: 100%ABR-T7AAC
    Expiration date: 7 Aug 2012

    I am very pleased to hear you would like to accept our offer and test ab123596 inrat. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Regarding your previous work with ab67017,I would like to encourage you to take advantage of our Abreview program. While we cannot offer the same 100% discount program as above, you may still collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. To leave and Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and clickSubmit an Abreview. You can gain more Abpoints by submitting images as well.

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Answer

    Thank you for contacting us.



    I have received a response from the lab regarding Ab117460 Anti-FCHSD1 antibody. The immunogen sequence is identical with that of rat, but we did not obtain a positive signal in rat tissues, so it is not listed as rat reactive.



    We do offer two other anti-FCHSD1 antibodies which have not been tested in rat. The homologies between the immunogens used to create these and the rat protein is over 90%. Therefore, I can offer a discount off a future purchase if you buy ab67017 or ab123596 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount code would be to the value of 1 free PRIMARY ANTIBODY.



    The product pages for those antibodies are :

    https://www.abcam.com/FCHSD1-antibody-ab67017.html

    https://www.abcam.com/FCHSD1-antibody-ab123596.html


    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test one or both of these products in rat. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

    2. Purchase either by phone, fax, or online (www.abcam.com).

    3. Test it in rat.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if this product turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

    Read More
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neuro2a cells)
    Loading amount
    20 µg
    Specification
    neuro2a cells
    Treatment
    FCHSD1 shRNA
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Aug 15 2011

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