Key features and details
- Rabbit polyclonal to FCHSD2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-FCHSD2 antibody
DescriptionRabbit polyclonal to FCHSD2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human pancreas tissue; RT 4, U 251 MG, Human liver and tonsil lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||1/250 - 1/500.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityLiver, brain, heart, placenta, skeletal muscle, pancreas, lung and kidney.
Sequence similaritiesContains 1 FCH domain.
Contains 2 SH3 domains.
- Information by UniProt
- Carom antibody
- FCH and double SH3 domains 2 antibody
- FCH and double SH3 domains protein 2 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab122485 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-FCHSD2 antibody (ab122485) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
ab122485, at 1/50, staining FCHSD2 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
ab122485 has not yet been referenced specifically in any publications.