Product nameAnti-FCHSD2 antibody
DescriptionRabbit polyclonal to FCHSD2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human pancreas tissue; RT 4, U 251 MG, Human liver and tonsil lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityLiver, brain, heart, placenta, skeletal muscle, pancreas, lung and kidney.
Sequence similaritiesContains 1 FCH domain.
Contains 2 SH3 domains.
- Information by UniProt
- Carom antibody
- FCH and double SH3 domains 2 antibody
- FCH and double SH3 domains protein 2 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, plasma membrane and cytoskeleton (actin filaments). Recommended concentration of ab122485 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-FCHSD2 antibody (ab122485) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
ab122485, at 1/50, staining FCHSD2 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
ab122485 has not yet been referenced specifically in any publications.