Overview

  • Product name

  • Description

    Rabbit polyclonal to FCP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-11 of Human FCP1.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2 and MOLT4 whole cell lysates; Human cal27 and HeLa xenograft tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab137683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 104 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer (pH8.0)

Target

  • Function

    Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II.
  • Tissue specificity

    Ubiquitously expressed. Isoform 3 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and placenta.
  • Involvement in disease

    Defects in CTDP1 are a cause of congenital cataracts facial dysmorphism and neuropathy syndrome (CCFDN) [MIM:604168]. CCFDN is an autosomal recessive developmental disorder that occurs in an endogamous group of Vlax Roma (Gypsies). The syndrome is characterized by a complex clinical phenotype with seemingly unrelated features involving multiple organs and systems. Developmental abnormalities include congenital cataracts and microcorneae, hypomyelination of the peripheral nervous system, impaired physical growth, delayed early motor and intellectual development, facial dysmorphism and hypogonadism. Central nervous system involvement, with cerebral and spinal cord atrophy, may be the result of disrupted development with superimposed degenerative changes. Affected individuals are prone to severe rhabdomyolysis after viral infections and to serious complications related to general anesthesia (such as pulmonary edema and epileptic seizures).
  • Sequence similarities

    Contains 1 BRCT domain.
    Contains 1 FCP1 homology domain.
  • Post-translational
    modifications

    Phosphorylated. In the presence of TFIIF, the phosphorylated form has an increased CTD phosphatase activity. The phosphorylation is required for the physical interaction with GTF2F1.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCFDN antibody
    • CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1 antibody
    • CTD of POLR2A, phosphatase of, subunit 1 antibody
    • CTDP1 antibody
    • CTDP1_HUMAN antibody
    • EC 3.1.3.16 antibody
    • RNA polymerase II subunit A C terminal domain phosphatase antibody
    • RNA polymerase II subunit A C-terminal domain phosphatase antibody
    • Serine phosphatase FCP1a antibody
    • TFIIF associating CTD phosphatase antibody
    • TFIIF-associating CTD phosphatase 1 antibody
    • TFIIF-associating CTD phosphatase antibody
    • Transcription factor IIF-associating CTD phosphatase 1 antibody
    see all

Images

  • All lanes : Anti-FCP1 antibody (ab137683) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 104 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labelling FCP1 with ab137683 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human HeLa xenograft tissue labelling FCP1 with ab137683 at 1/100 dilution.

References

This product has been referenced in:

  • Fu H  et al. Carboxy-terminal domain phosphatase 1 silencing results in the inhibition of tumor formation ability in gastric cancer cells. Oncol Lett 10:2947-2952 (2015). Read more (PubMed: 26722269) »
See 1 Publication for this product

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