Anti-FDFT1 antibody (ab236675)
Key features and details
- Rabbit polyclonal to FDFT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FDFT1 antibody
See all FDFT1 primary antibodies -
Description
Rabbit polyclonal to FDFT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human FDFT1 aa 1 to the C-terminus.
Database link: P37268 -
Positive control
- WB: HepG2, SH-SY5Y and HEK-293 cell lysate. IHC-P: Human adrenal gland tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 48 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 48 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Pathway
Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3. -
Sequence similarities
Belongs to the phytoene/squalene synthase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 281767 Cow
- Entrez Gene: 2222 Human
- Omim: 184420 Human
- SwissProt: Q32KR6 Cow
- SwissProt: P37268 Human
- Unigene: 593928 Human
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Alternative names
- DGPT antibody
- ERG9 antibody
- Farnesyl diphosphate farnesyltransferase antibody
see all
Images
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All lanes : Anti-FDFT1 antibody (ab236675) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa -
Paraffin-embedded human adrenal gland tissue stained for FDFT1 using ab236675 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FDFT1 using ab236675 at 1/100 dilution in ICC/IF. Secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236675 has not yet been referenced specifically in any publications.