Key features and details
- Rabbit polyclonal to FDFT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FDFT1 antibody
See all FDFT1 primary antibodies
DescriptionRabbit polyclonal to FDFT1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
Recombinant full length protein corresponding to Human FDFT1 aa 2-417.
EFVKCLGHPEEFYNLVRFRIGGKRKVMPKMDQDSLSSSLKTCYKYLNQTS RSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPLLHNFH SFLYQPDWRFMESKEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMG IGMAEFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDT ERANSMGLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPEN IDLAVQCLNELITNALHHIPDVITYLSRLRNQSVFNFCAIPQVMAIATLA ACYNNQQVFKGAVKIRKGQAVTLMMDATNMPAVKAIIYQYMEEIYHRIPD SDPSSSKTRQIISTIRTQNLPNCQLISRSHYSPIYLSFVMLLAALSWQYL TTLSQVTEDYVQTGEH
Database link: P37268
- WB: HepG2, SH-SY5Y and HEK-293 cell lysate. IHC-P: Human adrenal gland tissue. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab236675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 48 kDa.|
|ICC/IF||1/50 - 1/200.|
PathwayTerpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Sequence similaritiesBelongs to the phytoene/squalene synthase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- DGPT antibody
- ERG9 antibody
- Farnesyl diphosphate farnesyltransferase antibody
All lanes : Anti-FDFT1 antibody (ab236675) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Paraffin-embedded human adrenal gland tissue stained for FDFT1 using ab236675 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FDFT1 using ab236675 at 1/100 dilution in ICC/IF. Secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236675 has not yet been referenced specifically in any publications.