Overview

  • Product name

  • Description

    Rabbit polyclonal to FDFT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FDFT1 aa 100-150. The exact sequence is proprietary.
    Database link: P37268

  • Positive control

    • IP: HEK-293T whole cell lysate.
  • General notes

    ab245673 has not performed satisfactorily when used for WB of FDFT1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of FDFT1.

Properties

Applications

Our Abpromise guarantee covers the use of ab245673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Pathway

      Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
    • Sequence similarities

      Belongs to the phytoene/squalene synthase family.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • DGPT antibody
      • ERG9 antibody
      • Farnesyl diphosphate farnesyltransferase antibody
      • Farnesyl-diphosphate farnesyltransferase antibody
      • FDFT_HUMAN antibody
      • FDFT1 antibody
      • FPP:FPP farnesyltransferase antibody
      • SQS antibody
      • Squalene synthase antibody
      • Squalene synthetase antibody
      • SS antibody
      see all

    Images

    • FDFT1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
      ab245673 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

      Lane 1: ab245673 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    References

    ab245673 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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