Product nameAnti-FDFT1 antibody
See all FDFT1 primary antibodies
DescriptionRabbit polyclonal to FDFT1
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human FDFT1 aa 100-150. The exact sequence is proprietary.
Database link: P37268
- IP: HEK-293T whole cell lysate.
ab245673 has not performed satisfactorily when used for WB of FDFT1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of FDFT1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245673 was affinity purified using an epitope specific to FDFT1 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab245673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
PathwayTerpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Sequence similaritiesBelongs to the phytoene/squalene synthase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- DGPT antibody
- ERG9 antibody
- Farnesyl diphosphate farnesyltransferase antibody
FDFT1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245673 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.
Lane 1: ab245673 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Chemiluminescence detection: 30 seconds.
ab245673 has not yet been referenced specifically in any publications.