Overview

  • Product name

    Anti-FDFT1 antibody [EPR16481]
    See all FDFT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16481] to FDFT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FDFT1 aa 150-300. The exact sequence is proprietary.
    Database link: P37268

  • Positive control

    • Human fetal brain and fetal spleen lysate. HepG2 whole cell lysate (ab7900). Mouse brain, rat spleen, rat kidney and RAW264.7 cell lysate. Human lung squamous carcinoma tissue. Human sebaceous carcinoma tissue. A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/70.
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 48 kDa.
ICC/IF 1/250.

Target

Images

  • All lanes : Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 48 kDa

  • Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/10000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 48 kDa

  • All lanes : Anti-FDFT1 antibody [EPR16481] (ab195046) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : RAW264.7 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

  • Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human sebaceous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • FDFT1 was immunoprecipitated from HepG2 whole cell extract with ab195046 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab195046 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HepG2 whole cell extract (Input) 10 µg. Lane 2: ab195046 IP in HepG2 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195046 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

  • Volkmar N  et al. The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis. J Cell Sci 132:N/A (2019). Read more (PubMed: 30578317) »
  • Zhang P  et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med 23:1055-1062 (2017). Read more (PubMed: 28805822) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab195046.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up