Recombinant
RabMAb

Recombinant Anti-FDFT1 antibody [EPR16481] - BSA and Azide free (ab232552)

Overview

  • Product name

    Anti-FDFT1 antibody [EPR16481] - BSA and Azide free
    See all FDFT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16481] to FDFT1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human FDFT1 aa 150-300. The exact sequence is proprietary.
    Database link: P37268

  • Positive control

    • IHC-P: Human sebaceous carcinoma tissue.
  • General notes

    Ab232552 is the carrier-free version of ab195046. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232552 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195046).

  • FDFT1 was immunoprecipitated from HepG2 whole cell extract with ab195046 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab195046 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HepG2 whole cell extract (Input) 10 µg. Lane 2: ab195046 IP in HepG2 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195046 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195046).

  • Immunohistochemical analysis of paraffin-embedded human sebaceous carcinoma tissue sections labeling FDFT1 with ab195046 at a 1/16000 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195046).

References

ab232552 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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