Recombinant Anti-FDFT1 antibody [EPR4626] (ab109723)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-FDFT1 antibody [EPR4626]
See all FDFT1 primary antibodies -
Description
Rabbit monoclonal [EPR4626] to FDFT1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FDFT1. The exact sequence is proprietary.
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Positive control
- Fetal liver, HepG2, Molt-4, and SH-SY5Y lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4626 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Pathway
Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3. -
Sequence similarities
Belongs to the phytoene/squalene synthase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2222 Human
- Omim: 184420 Human
- SwissProt: P37268 Human
- Unigene: 593928 Human
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Alternative names
- DGPT antibody
- ERG9 antibody
- Farnesyl diphosphate farnesyltransferase antibody
see all
Images
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![Western blot - Anti-FDFT1 antibody [EPR4626] (ab109723)](https://www.abcam.com/ps/products/109/ab109723/Images/ab109723-122671-anti-fdft1-antibody-epr4626-western-blot.jpg)
Western blot - Anti-FDFT1 antibody [EPR4626] (ab109723)All lanes : Anti-FDFT1 antibody [EPR4626] (ab109723) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : Molt-4 lysate
Lane 4 : SH-SY5Y lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Volkmar N et al. The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis. J Cell Sci 132:N/A (2019). Read more (PubMed: 30578317) »
- Shimada K et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol 12:497-503 (2016). WB . Read more (PubMed: 27159577) »
