Anti-FDPS/FPS antibody (ab153805)
Key features and details
- Rabbit polyclonal to FDPS/FPS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FDPS/FPS antibody
See all FDPS/FPS primary antibodies -
Description
Rabbit polyclonal to FDPS/FPS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human FDPS/FPS aa 43-381.
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Positive control
- A549, HeLa, HepG2, HCT116 and mouse brain lysates; HeLa cells; Ca922 xenograft.
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General notes
Previously labelled as FDPS.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. -
Pathway
Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. -
Sequence similarities
Belongs to the FPP/GGPP synthase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281156 Cow
- Entrez Gene: 2224 Human
- Entrez Gene: 110196 Mouse
- Entrez Gene: 100153932 Pig
- Entrez Gene: 83791 Rat
- Omim: 134629 Human
- SwissProt: Q8WMY2 Cow
- SwissProt: P14324 Human
see all -
Alternative names
- (2E antibody
- (2E,6E) farnesyl diphosphate synthase antibody
- 6E)-farnesyl diphosphate synthase antibody
see all
Images
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Western blot - Anti-FDPS/FPS antibody (ab153805)Anti-FDPS/FPS antibody (ab153805) at 1/10000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Western blot - Anti-FDPS/FPS antibody (ab153805)Anti-FDPS/FPS antibody (ab153805) at 1/3000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 48 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FDPS/FPS antibody (ab153805)Immunofluorescent analysis of methanol-fixed HeLa cells labeling FDPS/FPS with ab153805 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDPS/FPS antibody (ab153805)Immunohistochemical analysis of paraffin-embedded Ca922 xenograft labeling FDPS/FPS with ab153805 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab153805 has been referenced in 4 publications.
- Pinner AL et al. Protein expression of prenyltransferase subunits in postmortem schizophrenia dorsolateral prefrontal cortex. Transl Psychiatry 10:3 (2020). PubMed: 32066669
- Fiore D et al. N6-Isopentenyladenosine Inhibits Colorectal Cancer and Improves Sensitivity to 5-Fluorouracil-Targeting FBXW7 Tumor Suppressor. Cancers (Basel) 11:N/A (2019). PubMed: 31569395
- Kim HY et al. Farnesyl diphosphate synthase is important for the maintenance of glioblastoma stemness. Exp Mol Med 50:137 (2018). PubMed: 30333528
- Du CQ et al. Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced fibrotic responses in vascular smooth muscle cells. Int J Mol Med 35:1767-72 (2015). PubMed: 25847782