Anti-FDPS/FPS antibody (ab226399)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FDPS/FPS antibody
See all FDPS/FPS primary antibodies -
Description
Rabbit polyclonal to FDPS/FPS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FDPS/FPS aa 50-100. The exact sequence is proprietary.
Database link: P14324 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
Previously labelled as FDPS.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226399 was affinity purified using an epitope specific to FDPS/FPS immobilized on solid support. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 48 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. -
Pathway
Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. -
Sequence similarities
Belongs to the FPP/GGPP synthase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2224 Human
- Omim: 134629 Human
- SwissProt: P14324 Human
- Unigene: 335918 Human
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Alternative names
- (2E antibody
- (2E,6E) farnesyl diphosphate synthase antibody
- 6E)-farnesyl diphosphate synthase antibody
see all
Images
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Western blot - Anti-FDPS/FPS antibody (ab226399)All lanes : Anti-FDPS/FPS antibody (ab226399) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer
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Immunoprecipitation - Anti-FDPS/FPS antibody (ab226399)FDPS/FPS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226399 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226399 at 1 µg/ml.
Lane 1: ab226399 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab226399 has not yet been referenced specifically in any publications.
