Overview

  • Product name

  • Description

    Goat polyclonal to FE65
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Peptide with sequence CGSLKPKRLGAHTP, from the C Terminus of the protein sequence (Human)

  • Positive control

    • NIH/3T3 lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/128000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (observed in lysates of NIH/3T3 and HeLa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
    • Tissue specificity

      Highly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
    • Sequence similarities

      Contains 2 PID domains.
      Contains 1 WW domain.
    • Post-translational
      modifications

      Phosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1.
    • Cellular localization

      Cell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adaptor protein FE65a2 antibody
      • Amyloid beta (A4) precursor protein binding family B member 1 antibody
      • Amyloid beta A4 precursor protein binding family B antibody
      • Amyloid beta A4 precursor protein binding family B member 1 antibody
      • Amyloid beta A4 precursor protein-binding family B member 1 antibody
      • Amyloid beta precursor protein binding family B member 1 antibody
      • APBB 1 antibody
      • APBB1 antibody
      • APBB1_HUMAN antibody
      • FE 65 antibody
      • Fe65 protein antibody
      • Protein Fe65 antibody
      • RIR antibody
      • stat like protein antibody
      see all

    Images

    References

    ab51980 has not yet been referenced specifically in any publications.

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