Key features and details
- Goat polyclonal to FE65
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FE65 antibody
See all FE65 primary antibodies
DescriptionGoat polyclonal to FE65
SpecificityThis antibody is expected to recognise both reported isoforms.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Peptide with sequence CGSLKPKRLGAHTP, from the C Terminus of the protein sequence (Human)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab51980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (observed in lysates of NIH/3T3 and HeLa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
Tissue specificityHighly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
Sequence similaritiesContains 2 PID domains.
Contains 1 WW domain.
modificationsPhosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1.
Cellular localizationCell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus.
- Information by UniProt
- Adaptor protein FE65a2 antibody
- Amyloid beta (A4) precursor protein binding family B member 1 antibody
- Amyloid beta A4 precursor protein binding family B antibody
Anti-FE65 antibody (ab51980) at 0.1 µg/ml + NIH/3T3 lysate in RIPA buffer at 35 µg
Observed band size: 100 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab51980 has not yet been referenced specifically in any publications.