Product nameAnti-FE65 antibody [EPR3538]
See all FE65 primary antibodies
DescriptionRabbit monoclonal [EPR3538] to FE65
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues in human FE65 (UniProt ID: O00213).
- SH-SY5Y cell lysate and Human brain tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab91650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody. We have compared both the HRP-conjugated and the polymerized HRP and found stronger signals can be obtained with the polymerized antibody.
WB: 1/2000 - 1/5000. Predicted molecular weight: 77 kDa.
Is unsuitable for Flow Cyt or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
Tissue specificityHighly expressed in brain; strongly reduced in post-mortem elderly subjects with Alzheimer disease.
Sequence similaritiesContains 2 PID domains.
Contains 1 WW domain.
modificationsPhosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1.
Cellular localizationCell membrane. Cytoplasm. Nucleus. Cell projection > growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus.
- Information by UniProt
- Adaptor protein FE65a2 antibody
- Amyloid beta (A4) precursor protein binding family B member 1 antibody
- Amyloid beta A4 precursor protein binding family B antibody
Anti-FE65 antibody [EPR3538] (ab91650) at 1/2000 dilution + SH-SY5Ycell
lysates at 10 µg
HRP labelled goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 77 kDa
ab91650, at 1/50 dilution, staining FE65 in formalin-fixed, paraffin-embedded Human brain tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.