Overview

  • Product name

    Anti-Feline Calicivirus antibody [FCV1-43]
  • Description

    Mouse monoclonal [FCV1-43] to Feline Calicivirus
  • Host species

    Mouse
  • Specificity

    ab33990 specifically recognises feline calicivirus, and does not cross-react with FIV or FeLV.
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    The antibody detects a 63kD band in Western blots, representing the viral capsid protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    The antibody detects a 63kD band in Western blots, representing the viral capsid protein.
  • Clonality

    Monoclonal
  • Clone number

    FCV1-43
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 63 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Feline calicivirus is a common viral disease that affects cats. It is characterized by upper respiratory symptoms, pneumonia, oral ulceration, and occasionally arthritis. It is a fairly mild flu-like condition and rarely causes serious complications. FCV is a tiny non-enveloped virus consisting of a 20 sided protein capsid (that fashions cup like dimples) surrounding a single strand of positive sense RNA.
  • Database links

    • Alternative names

      • FCV antibody

    References

    This product has been referenced in:

    • Yamashiro R  et al. Key role of singlet oxygen and peroxynitrite in viral RNA damage during virucidal effect of plasma torch on feline calicivirus. Sci Rep 8:17947 (2018). Read more (PubMed: 30560882) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    I was able to obtain the following information:

    Not only does this antibody bind the 63kDa capsid protein, but it has also recently been shown to specifically recognize the vp2 region by binding to protein expressed by a construct specifically encoding vp2. The epitope recognized by this antibody is believed to be both linear and containing no glycosylation sites.

    We were not able to obtain an image of the blot though, or information about specificity for strains of FCV.

    Read More

    Answer

    I have forwarded your data to the lab and I have asked if there is any problem with the lot you have.

    I am assuming the QC test that they did confirmed reactivity with FCV, and that the sample did not contain FBS or serum of any kind. In any case, I think the best approach now is to see what results you get with ab93284.

    It is possible that the vial of ab33990 you have now was defective when you received it and that it will not detect FCV of any strain. On the other hand, it is specific for a single epitope on the capsid, so there is the possibility that the capsid you are working with does not contain this epitope, if there is capsid variation among FCV strains.

    Read More

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