Anti-FEN1 antibody (ab153825)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-FEN1 antibody
See all FEN1 primary antibodies -
Description
Rabbit polyclonal to FEN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 169 and 380 of Human FEN1 (UniProt: P39748).
-
Positive control
- Recombinant Human FEN1 protein (ab95382) can be used as a positive control in WB. HepG2, NIH 3T3, JC, BCL1, Raw264.7 and PC12 whole cell lysates; A549 cells; A549 xenograft tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 43 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. -
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. -
Post-translational
modificationsAcetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. - Information by UniProt
-
Database links
- Entrez Gene: 2237 Human
- Entrez Gene: 14156 Mouse
- Entrez Gene: 84490 Rat
- Omim: 600393 Human
- SwissProt: P39748 Human
- SwissProt: P39749 Mouse
- SwissProt: Q5XIP6 Rat
- SwissProt: Q9JHW7 Rat
see all -
Alternative names
- DNase IV antibody
- FEN-1 antibody
- FEN1 antibody
see all
Images
-
Western blot - Anti-FEN1 antibody (ab153825)Anti-FEN1 antibody (ab153825) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Western blot - Anti-FEN1 antibody (ab153825)All lanes : Anti-FEN1 antibody (ab153825) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : Raw264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS-PAGE -
Western blot - Anti-FEN1 antibody (ab153825)Anti-FEN1 antibody (ab153825) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (ab153825)Immunofluorescence analysis of paraformaldehyde fixed A549 cells labeling FEN1 with ab153825 at 1/200. The lower panel is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (ab153825)Immunohistochemical analysis of paraffin embedded A549 xenograft tissue labeling FEN1 with ab153825 at 1/100.
Protocols
References
ab153825 has not yet been referenced specifically in any publications.
