Recombinant
RabMAb

Recombinant Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)

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Overview

  • Product name

    Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free
    See all FEN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4460(2)] to FEN1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FEN1 aa 300-400. The exact sequence is proprietary.

  • General notes

    Ab239948 is the carrier-free version of ab109132. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239948 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4460(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab239948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

    • Sequence similarities

      Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

    • Post-translational
      modifications

      Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
      Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
      Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

    • Cellular localization

      Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DNase IV antibody
      • FEN-1 antibody
      • FEN1 antibody
      • FEN1_HUMAN antibody
      • Flap endonuclease 1 antibody
      • Flap structure specific endonuclease 1 antibody
      • Flap structure-specific endonuclease 1 antibody
      • hFEN-1 antibody
      • hFEN1 antibody
      • Maturation factor 1 antibody
      • MF1 antibody
      • Rad2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109132 at a dilution of 1/500.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109132).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109132 at a dilution of 1/500.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109132).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free (ab239948)

      Immunohistochemical analysis of paraffin-embedded Human urothelial carcinoma tissue using ab109132 at a dilution of 1/500.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109132).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab239948 has not yet been referenced specifically in any publications.

    Publishing research using ab239948? Please let us know so that we can cite the reference in this datasheet.

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