Overview

  • Product name

  • Description

    Rabbit polyclonal to FER
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide corresponding to Human FER aa 521-536 (internal sequence).
    Sequence:

    FSNIPQLIDHHYTTKQ


    Database link: P16591

  • Positive control

    • Rat intestine tissue; Human intestinal cancer tissue; Rat testis and ovary tissue lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 95 kDa.

The detection limit for FER is approximately 5ng/lane under reducing conditions.

Target

  • Function

    Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
  • Tissue specificity

    Expressed in a variety of lymphoid cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
    Contains 1 FCH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • Cellular localization

    Cytoplasm. Nucleus. Associated with the chromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • c FER antibody
    • Feline encephalitis virus related kinase FER antibody
    • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
    • Fer (fps/fes related) tyrosine kinase antibody
    • FER antibody
    • FER_HUMAN antibody
    • FerT antibody
    • Fujinami poultry sarcoma/Feline sarcoma related protein Fer antibody
    • p94 FER antibody
    • p94-FER antibody
    • Pe1Fe10 antibody
    • Pe1Fe13 antibody
    • Pe1Fe3 antibody
    • Pe1Fe6 antibody
    • Phosphoprotein NCP94 antibody
    • PPP1R74 antibody
    • Protein phosphatase 1 regulatory subunit 74 antibody
    • Proto oncogene tyrosine protein kinase FER antibody
    • Proto-oncogene c-Fer antibody
    • TYK3 antibody
    • Tyrosine kinase 3 antibody
    • Tyrosine-protein kinase Fer antibody
    see all

Images

  • All lanes : Anti-FER antibody (ab191060) at 0.5 µg/ml

    Lane 1 : Rat testis tissue lysate
    Lane 2 : Rat ovary tissue lysate

    Predicted band size: 95 kDa

  • Immunohistochemical analysis of paraffin-embedded rat intestine tissue labeling FER with ab191060 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue labeling FER with ab191060 at 1 μg/ml.

References

This product has been referenced in:

  • Dolgachev V  et al. Electroporation-mediated delivery of FER gene enhances innate immune response and improves survival in a murine model of pneumonia. Gene Ther N/A:N/A (2018). Read more (PubMed: 29907877) »
  • Dolgachev VA  et al. Electroporation-mediated delivery of the FER gene in the resolution of trauma-related fatal pneumonia. Gene Ther 23:785-796 (2016). Read more (PubMed: 27454317) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191060.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up