Anti-FER (phospho Y402) antibody (ab79573)
Key features and details
- Rabbit polyclonal to FER (phospho Y402)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FER (phospho Y402) antibody
See all FER primary antibodies -
Description
Rabbit polyclonal to FER (phospho Y402) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human FER (phospho Y402).
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Positive control
- Human brain tissue; Jurkat cell extracts starved for 24 hours.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 93 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 93 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control. -
Tissue specificity
Expressed in a variety of lymphoid cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
Cellular localization
Cytoplasm. Nucleus. Associated with the chromatin. - Information by UniProt
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Database links
- Entrez Gene: 2241 Human
- Entrez Gene: 14158 Mouse
- Omim: 176942 Human
- SwissProt: P16591 Human
- SwissProt: P70451 Mouse
- Unigene: 221472 Human
- Unigene: 23039 Mouse
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Alternative names
- c FER antibody
- Feline encephalitis virus related kinase FER antibody
- Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
see all
Images
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ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
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All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution
Lane 1 : Jurkat cell extracts, starved (24 hours)
Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab79573 has been referenced in 2 publications.
- Amtul Z et al. Comorbid rat model of ischemia and β-amyloid toxicity: striatal and cortical degeneration. Brain Pathol 25:24-32 (2015). PubMed: 24725245
- Chang SF et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A 111:1855-60 (2014). PubMed: 24449884