Overview

  • Product name

  • Description

    Rabbit polyclonal to FERDL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: MAAYPESCVD TTVLDFVADL SLASPRRPLL CDFAPGVSLG DPALALREGR PRRMARFEEG DPEEEECEVD Q, corresponding to amino acids 1-71 of Human FERDL3 (Q96RJ6)

  • Positive control

    • Human colon tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function

    Transcription factor that binds to the E-box and functions as inhibitor of transcription. DNA binding requires dimerization with an E protein. Inhibits transcription activation by ASCL1/MASH1 by sequestering E proteins.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic helix-loop-helix protein N-twist antibody
    • bHLHa31 antibody
    • Class A basic helix-loop-helix protein 31 antibody
    • Fer3 like (Drosophila) antibody
    • Fer3-like protein antibody
    • FER3L_HUMAN antibody
    • Ferd3l antibody
    • MGC119861 antibody
    • N TWIST antibody
    • NATO3 antibody
    • Nephew of atonal 3 antibody
    • Neuronal twist antibody
    • NTWIST antibody
    see all

Images

  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab126381, at 1/50 dilution, staining FERDL3 in paraffin-embedded Human colon tissue by Immunohistochemistry.

References

ab126381 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your interest.
The sequence homology between human and mouse is relatively low so we do not expect cross-reactivity with murine FERDL3. Please see the Blast search below:
fer3-like protein [Mus musculus]
Sequence ID: gi|15743552|ref|NP_277057.1|Length: 168Number of Matches: 1
See 5 more title(s)RecName: Full=Fer3-like protein; AltName: Full=Basic helix-loop-helix protein N-twist; AltName: Full=Nephew of atonal 3; AltName: Full=Neuronal twist
Sequence ID: gi|81902671|sp|Q923Z4.1|FER3L_MOUSELength: 168Number of Matches:
Fer3-like [Mus musculus]
Sequence ID: gi|14718588|gb|AAK72955.1|AF369896_1Length: 168Number of Matches:
N-TWIST basic helix-loop-helix protein [Mus musculus]
Sequence ID: gi|22655501|gb|AAN04085.1|Length: 168Number of Matches:
Nephew of atonal 3 [Mus musculus]
Sequence ID: gi|84105445|gb|AAI11577.1|Length: 168Number of Matches:
Fer3-like (Drosophila) [Mus musculus]
Sequence ID: gi|148704925|gb|EDL36872.1|Length: 168Number of Matches: Related Information
Gene-associated gene details
UniGene-clustered expressed sequence tags
Map Viewer-aligned genomic contextRange 1: 1 to 65GenPeptGraphics Next Match Previous Match First Match Alignment statistics for match #1 Score Expect Method Identities Positives Gaps Frame
94.8 bits(216) 3e-19() Compositional matrix adjust. 44/65(68%) 48/65(73%) 0/65(0%)
Features:
If you need any further assistance in the future, please do not hesitate to contact me.

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