Anti-Ferredoxin-NADP reductase antibody (ab243189)
Key features and details
- Rabbit polyclonal to Ferredoxin-NADP reductase
- Suitable for: WB
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
Overview
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Product name
Anti-Ferredoxin-NADP reductase antibody -
Description
Rabbit polyclonal to Ferredoxin-NADP reductase -
Host species
Rabbit -
Specificity
This antibody reacts with plant root FNR proteins (R-FNR1 and R-FNR2) including Arabidopsis and Maize. It also reacts with leaf FNRs.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Arabidopsis thaliana -
Immunogen
Recombinant full length protein corresponding to Ferredoxin-NADP reductase. Maize. Purified.
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Positive control
- WB: Extracts from wild type strain Arabidopsis Col, mutant rfer1 and rfnr2-2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Filter sterilized -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab243189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/30000. |
Images
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Western blot - Anti-Ferredoxin-NADP reductase antibody (ab243189)All lanes : Anti-Ferredoxin-NADP reductase antibody (ab243189) at 1/2000 dilution
Lanes 1 & 3 : Extracts from wild type strain Arabidopsis Col
Lane 2 : Extracts from wild type strain Arabidopsis mutant rfer1
Lane 4 : Extracts from wild type strain Arabidopsis mutant rfnr2-2Note that R-FNR2 is the dominant form in wild-type roots. Wild-type produces both R-FNR1 and R-FNR2. Mutant rfnr1 produces R-FNR2 and mutant rfr2 produces R-FNR1.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (2)
ab243189 has been referenced in 2 publications.
- Hachiya T et al. Arabidopsis Root-Type Ferredoxin:NADP(H) Oxidoreductase 2 is Involved in Detoxification of Nitrite in Roots. Plant Cell Physiol 57:2440-2450 (2016). WB . PubMed: 27615794
- Onda Y et al. Differential interaction of maize root ferredoxin:NADP(+) oxidoreductase with photosynthetic and non-photosynthetic ferredoxin isoproteins. Plant Physiol 123:1037-45 (2000). WB . PubMed: 10889253
