Anti-Ferredoxin Reductase antibody (ab204310)
Key features and details
- Rabbit polyclonal to Ferredoxin Reductase
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ferredoxin Reductase antibody
See all Ferredoxin Reductase primary antibodies -
Description
Rabbit polyclonal to Ferredoxin Reductase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Ferredoxin Reductase aa 1-150.
Database link: P22570 -
Positive control
- IHC-P: Human adrenal gland, testis tissue. ICC: U-2 OS cells. WB: A549, RT4, and U-251 MG cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa.
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa. |
ICC
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. -
Pathway
Steroid metabolism; cholesterol metabolism. -
Sequence similarities
Belongs to the ferredoxin--NADP reductase type 1 family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 2232 Human
- Omim: 103270 Human
- SwissProt: P22570 Human
- Unigene: 69745 Human
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Alternative names
- Adrenodoxin reductase antibody
- ADRO_HUMAN antibody
- ADXR antibody
see all
Images
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All lanes : Anti-Ferredoxin Reductase antibody (ab204310) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) cell lysate
Predicted band size: 54 kDa -
Immunocytochemistry analysis of U-2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling Ferredoxin Reductase with ab204310 at 2 µg/mL.
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Paraffin embedded human adrenal gland tissue stained for Ferredoxin Reductase using ab204310 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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All lanes : Anti-Ferredoxin Reductase antibody (ab204310) at 0.4 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) cell lysate
Predicted band size: 54 kDa -
Paraffin embedded human testis tissue stained for Ferredoxin Reductase using ab204310 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human liver tissue stained for Ferredoxin Reductase using ab204310 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab204310 has been referenced in 4 publications.
- Yang L et al. Systemic Delivery of AAV-Fdxr Mitigates the Phenotypes of Mitochondrial Disorders in Fdxr Mutant Mice. Mol Ther Methods Clin Dev 18:84-97 (2020). PubMed: 32995353
- Clarke SL et al. Distinct TP53 Mutation Subtypes Differentially Influence Cellular Iron Metabolism. Nutrients 11:N/A (2019). PubMed: 31500291
- Peng Y et al. Biallelic mutations in the ferredoxin reductase gene cause novel mitochondriopathy with optic atrophy. Hum Mol Genet N/A:N/A (2018). PubMed: 29554255
- Peng Y et al. Biallelic mutations in the ferredoxin reductase gene cause novel mitochondriopathy with optic atrophy. Hum Mol Genet 26:4937-4950 (2017). PubMed: 29040572