Recombinant Anti-Ferritin antibody [EPR3004Y] (ab75973)
Rabbit recombinant monoclonal Ferritin antibody [EPR3004Y]. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 26 publication(s). Independently reviewed in 3 review(s).
- Datasheet
- References (31)
- Protocols
Overview
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Product name
Anti-Ferritin antibody [EPR3004Y]
See all Ferritin primary antibodies -
Description
Rabbit monoclonal [EPR3004Y] to Ferritin -
Host species
Rabbit -
Specificity
In human, this antibody recognizes both, Ferritin heavy and light chains. In mouse and rat, it only recognizes Ferritin heavy chain.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Frmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Ferritin aa 50-150. The exact sequence is proprietary.
Database link: P02794 -
Positive control
- WB: Jurkat, SH-SY-5Y, C6, Raw264.7, human and rat brain lysates ICC/IF: Jurkat cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3004Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab75973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21,19 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 25498860 |
Target
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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Database links
- Entrez Gene: 2495 Human
- Entrez Gene: 2512 Human
- Entrez Gene: 14319 Mouse
- Entrez Gene: 14325 Mouse
- Entrez Gene: 25319 Rat
- Entrez Gene: 29292 Rat
- Omim: 608847 Human
- SwissProt: P02792 Human
see all -
Form
Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. -
Alternative names
- Cell proliferation-inducing gene 15 protein antibody
- Ferritin H subunit antibody
- Ferritin heavy chain antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Ferritin antibody [EPR3004Y] (ab75973)](https://www.abcam.com/ps/products/75/ab75973/Images/ab75973-2-ab75973ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ferritin antibody [EPR3004Y] (ab75973)Immunofluorescence staining of Jurkat cells with purified ab75973 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75973 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)](https://www.abcam.com/ps/products/75/ab75973/Images/ab75973-256963-ab75973WB2.jpg)
Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/2000 dilution (purified)
Lane 1 : Rat brain lysate
Lane 2 : C6 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21,19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)](https://www.abcam.com/ps/products/75/ab75973/Images/ab75973-256964-ab75973WB3.jpg)
Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/10000 dilution (purified) + Raw264.7 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21,19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)](https://www.abcam.com/ps/products/75/ab75973/Images/ab75973-256962-ab75973WB1.jpg)
Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/10000 dilution (purified)
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21,19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)](https://www.abcam.com/ps/products/75/ab75973/Images/ab75973.jpg)
Western blot - Anti-Ferritin antibody [EPR3004Y] (ab75973)All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/2000 dilution (Unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY-5Y cell lysate
Lane 3 : Human brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21,19 kDa
Observed band size: 19,21 kDa why is the actual band size different from the predicted?
Protocols
References
This product has been referenced in:
- Zhang L et al. Role of mitochondrial calcium uniporter-mediated Ca2+ and iron accumulation in traumatic brain injury. J Cell Mol Med 23:2995-3009 (2019). Read more (PubMed: 30756474) »
- Yu X et al. Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells. Nutrients 11:N/A (2019). Read more (PubMed: 30813537) »
