Recombinant
RabMAb

Recombinant Anti-Ferritin antibody [EPR3004Y] (ab75973)

Rabbit recombinant monoclonal Ferritin antibody [EPR3004Y]. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 26 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name

    Anti-Ferritin antibody [EPR3004Y]
    See all Ferritin primary antibodies
  • Description

    Rabbit monoclonal [EPR3004Y] to Ferritin
  • Host species

    Rabbit
  • Specificity

    In human, this antibody recognizes both, Ferritin heavy and light chains. In mouse and rat, it only recognizes  Ferritin heavy chain. 

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ferritin aa 50-150. The exact sequence is proprietary.
    Database link: P02794

  • Positive control

    • WB: Jurkat, SH-SY-5Y, C6, Raw264.7, human and rat brain lysates ICC/IF: Jurkat cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21,19 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 25498860
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
    • Sequence similarities

      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt
    • Database links

    • Form

      Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
    • Alternative names

      • Cell proliferation-inducing gene 15 protein antibody
      • Ferritin H subunit antibody
      • Ferritin heavy chain antibody
      • Ferritin heavy polypeptide 1 antibody
      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • Ferritin, heavy polypeptide antibody
      • FHC antibody
      • FRIH_HUMAN antibody
      • FTH antibody
      • FTH1 antibody
      • FTHL6 antibody
      • FTL antibody
      • MGC104426 antibody
      • PIG15 antibody
      • PLIF antibody
      • Proliferation inducing protein 15 antibody
      see all

    Images

    • Immunofluorescence staining of Jurkat cells with purified ab75973 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75973 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/2000 dilution (purified)

      Lane 1 : Rat brain lysate
      Lane 2 : C6 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21,19 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/10000 dilution (purified) + Raw264.7 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21,19 kDa
      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/10000 dilution (purified)

      Lane 1 : SH-SY5Y whole cell lysate
      Lane 2 : Jurkat whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21,19 kDa
      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Ferritin antibody [EPR3004Y] (ab75973) at 1/2000 dilution (Unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY-5Y cell lysate
      Lane 3 : Human brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 21,19 kDa
      Observed band size: 19,21 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Zhang L  et al. Role of mitochondrial calcium uniporter-mediated Ca2+ and iron accumulation in traumatic brain injury. J Cell Mol Med 23:2995-3009 (2019). Read more (PubMed: 30756474) »
    • Yu X  et al. Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells. Nutrients 11:N/A (2019). Read more (PubMed: 30813537) »
    See all 31 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Eye, muscle)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel, NuPAGE)
    Loading amount
    23 µg
    Specification
    Eye, muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Cheri Ackerman

    Verified customer

    Submitted Aug 11 2016

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (TGX any size gel)
    Sample
    Fish Cell lysate - whole cell (Liver)
    Specification
    Liver
    Blocking step
    (agent) for 20 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

    Don Kuhn

    Verified customer

    Submitted Oct 29 2013

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Loading amount
    20 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Oct 06 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up