Overview

  • Product name

    Anti-Ferritin antibody [EPR3004Y] (HRP)
    See all Ferritin primary antibodies
  • Description

    Rabbit monoclonal [EPR3004Y] to Ferritin (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Ferritin aa 50-150. The exact sequence is proprietary.
    Database link: P02794

  • Positive control

    • WB: SHSY-5Y whole cell and human brain tissue lysates. IHC-P: normal human liver tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21,19 kDa).

Target

  • Function

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Sequence similarities

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Database links

  • Form

    Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
  • Alternative names

    • Cell proliferation-inducing gene 15 protein antibody
    • Ferritin H subunit antibody
    • Ferritin heavy chain antibody
    • Ferritin heavy polypeptide 1 antibody
    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • Ferritin, heavy polypeptide antibody
    • FHC antibody
    • FRIH_HUMAN antibody
    • FTH antibody
    • FTH1 antibody
    • FTHL6 antibody
    • FTL antibody
    • MGC104426 antibody
    • PIG15 antibody
    • PLIF antibody
    • Proliferation inducing protein 15 antibody
    see all

Images

  • Anti-Ferritin antibody [EPR3004Y] (HRP) (ab199256) at 1/5000 dilution + Brain (Human) Tissue Lysate - adult normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21,19 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199256 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Anti-Ferritin antibody [EPR3004Y] (HRP) (ab199256) at 1/5000 dilution + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21,19 kDa
    Observed band size: 21 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199256 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Ferritin staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab199256 at 1/100 dilution for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab199256 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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