Recombinant
RabMAb

Recombinant Anti-Ferritin antibody [EPR3004Y] - Low endotoxin, Azide free (ab215982)

Overview

  • Product name

    Anti-Ferritin antibody [EPR3004Y] - Low endotoxin, Azide free
    See all Ferritin primary antibodies
  • Description

    Rabbit monoclonal [EPR3004Y] to Ferritin - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ferritin aa 50-150. The exact sequence is proprietary.
    Database link: P02794

  • Positive control

    • WB: Jurkat, SH-SY-5Y, C6, Raw264.7, human and rat brain lysates ICC/IF: Jurkat cells
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 19, 21 kDa (predicted molecular weight: 21,19 kDa).
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 25498860
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
    • Sequence similarities

      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt
    • Database links

    • Form

      Subunit structure: Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.
    • Alternative names

      • Cell proliferation-inducing gene 15 protein antibody
      • Ferritin H subunit antibody
      • Ferritin heavy chain antibody
      • Ferritin heavy polypeptide 1 antibody
      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • Ferritin, heavy polypeptide antibody
      • FHC antibody
      • FRIH_HUMAN antibody
      • FTH antibody
      • FTH1 antibody
      • FTHL6 antibody
      • FTL antibody
      • MGC104426 antibody
      • PIG15 antibody
      • PLIF antibody
      • Proliferation inducing protein 15 antibody
      see all

    Images

    • Immunofluorescence staining of Jurkat cells with purified ab75973 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75973 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75973).

    References

    This product has been referenced in:

    • Heath JL  et al. Effects of iron depletion on CALM-AF10 leukemias. Exp Hematol 42:1022-30.e1 (2014). Read more (PubMed: 25193880) »
    • Naumova AV  et al. Magnetic Resonance Imaging Tracking of Graft Survival in the Infarcted Heart: Iron Oxide Particles Versus Ferritin Overexpression Approach. J Cardiovasc Pharmacol Ther N/A:N/A (2014). Mouse . Read more (PubMed: 24685664) »
    See all 4 Publications for this product

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