Overview

  • Product name

    Anti-Ferritin Heavy Chain antibody [3F8]
    See all Ferritin Heavy Chain primary antibodies
  • Description

    Mouse monoclonal [3F8] to Ferritin Heavy Chain
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, ChIPmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Human Ferritin Heavy Chain.
    Database link: P02794

  • Positive control

    • U87mg or HeLa cells
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR3175804 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab77127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ChIP Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • Apoferritin antibody
    • Cell proliferation inducing gene 15 protein antibody
    • Cell proliferation-inducing gene 15 protein antibody
    • F HC antibody
    • Ferritin H subunit antibody
    • Ferritin heavy chain antibody
    • Ferritin heavy polypeptide 1 antibody
    • FHC antibody
    • FRIH antibody
    • FRIH_HUMAN antibody
    • FTH 1 antibody
    • FTH antibody
    • FTH1 antibody
    • FTH1 protein antibody
    • FTHL 6 antibody
    • FTHL6 antibody
    • Iron overload autosomal dominant antibody
    • MGC104426 antibody
    • N-terminally processed antibody
    • OK/SW-cl.84 antibody
    • PIG 15 antibody
    • PIG15 antibody
    • Placenta immunoregulatory factor antibody
    • PLIF antibody
    • Proliferation inducing gene 15 protein antibody
    • Proliferation inducing protein 15 antibody
    see all

Images

  • All lanes : Anti-Ferritin Heavy Chain antibody [3F8] (ab77127) at 1/1000 dilution

    Lane 1 : U87mg cell lysate
    Lane 2 : HeLa cell lysate

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

References

This product has been referenced in:

  • Broide E  et al. Expression of Duodenal Iron Transporter Proteins in Diabetic Patients with and without Iron Deficiency Anemia. J Diabetes Res 2018:7494821 (2018). Read more (PubMed: 29693022) »
  • Chen X  et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). Read more (PubMed: 28858244) »
See all 2 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (brain)
Permeabilization
Yes - 0.3% Triton x 100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 08 2019

This product is known to not work in this application or species.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - TritonX 100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 08 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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