Overview

  • Product name

    Anti-Ferritin Light Chain antibody [8E1E7]
    See all Ferritin Light Chain primary antibodies
  • Description

    Mouse monoclonal [8E1E7] to Ferritin Light Chain
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Ferritin Light Chain.
    Database link: P02792

  • Positive control

    • Recombinant Ferritin Light Chain protein, Ferritin Light Chain transfected HEK293 cell lysate, K562 cell lysate, A431 cells. Human liver cancer tissue and esophageal tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab201975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease

    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • FRIL_HUMAN antibody
    • FTL antibody
    • LFTD antibody
    • NBIA 3 antibody
    • NBIA3 antibody
    see all

Images

  • Anti-Ferritin Light Chain antibody [8E1E7] (ab201975) at 1/500 dilution + recombinant human Ferritin Light Chain protein

    Predicted band size: 20 kDa

  • All lanes : Anti-Ferritin Light Chain antibody [8E1E7] (ab201975) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Ferritin Light Chain transfected HEK293 cell lysate

    Predicted band size: 20 kDa

  • Anti-Ferritin Light Chain antibody [8E1E7] (ab201975) at 1/500 dilution + K562 cell lysate

    Predicted band size: 20 kDa

  • Immunofluorescence analysis of A431 cells labeling Ferritin Light Chain using ab201975 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. 

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues labeling Ferritin Light Chain using ab201975 at 1/200 dilution with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Human esophageal tissue labeling Ferritin Light Chain with ab201975 at 1/200 dilution with DAB staining

References

ab201975 has not yet been referenced specifically in any publications.

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