Anti-Ferritin Light Chain antibody (ab110017)
Key features and details
- Goat polyclonal to Ferritin Light Chain
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ferritin Light Chain antibody
See all Ferritin Light Chain primary antibodies -
Description
Goat polyclonal to Ferritin Light Chain -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig -
Immunogen
Synthetic peptide:
C-GEYLFERLTLKHD
, corresponding to C terminal amino acids 163-175 of Human Ferritin Light Chain -
Positive control
- Human brain (cortex) tissue, Human kidney tissue, Human placenta lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
Involvement in disease
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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Database links
- Entrez Gene: 286861 Cow
- Entrez Gene: 477042 Dog
- Entrez Gene: 2512 Human
- Entrez Gene: 14325 Mouse
- Entrez Gene: 29292 Rat
- Omim: 134790 Human
- SwissProt: O46415 Cow
- SwissProt: Q53VB8 Dog
see all -
Alternative names
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
Images
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ab110017, at 3.75 µg/ml, staining Ferritin Light Chain in Human brain (cortex) by immunohistochemistry.
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ab110017, at 3.75 µg/ml, staining Ferritin Light Chain in Human kidney tissue by immunohistochemistry.
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Anti-Ferritin Light Chain antibody (ab110017) at 0.1 µg/ml + Human placenta lysate at 35 µg
Predicted band size: 20 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab110017 has been referenced in 1 publication.
- Gal J et al. Detergent Insoluble Proteins and Inclusion Body-Like Structures Immunoreactive for PRKDC/DNA-PK/DNA-PKcs, FTL, NNT, and AIFM1 in the Amygdala of Cognitively Impaired Elderly Persons. J Neuropathol Exp Neurol 77:21-39 (2018). PubMed: 29186589