Key features and details
- Rabbit polyclonal to Ferritin Light Chain
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Ferritin Light Chain antibody
See all Ferritin Light Chain primary antibodies
DescriptionRabbit polyclonal to Ferritin Light Chain
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Cat, Dog
Recombinant fragment, corresponding to a region within amino acids 1-175 of Human Ferritin Light Chain (UniProt ID: P02792).
- 293T, HepG2 and NIH3T3 whole cell lysates; Human A549 xenograft tissue
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 20 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Involvement in diseaseDefects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
Sequence similaritiesBelongs to the ferritin family.
Contains 1 ferritin-like diiron domain.
- Information by UniProt
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
Anti-Ferritin Light Chain antibody (ab153976) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 20 kDa
12% SDS PAGE
Anti-Ferritin Light Chain antibody (ab153976) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 20 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue labeling Ferritin Light Chain with ab153976 at 1/500 dilution.
ab153976 has not yet been referenced specifically in any publications.