Product nameAnti-Ferritin Light Chain antibody [CPT-R10-58-11]
See all Ferritin Light Chain primary antibodies
DescriptionRabbit monoclonal [CPT-R10-58-11] to Ferritin Light Chain
Tested applicationsSuitable for: WB, Flow Cyt, ELISAmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Ferritin Light Chain aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P02792
- WB: NCI-H460, HEK-293T, MCF7 and HepG2 whole cell lysates; Human brain tissue lysate. Flow Cyt: HepG2 cells. ELISA: CPTAC-14d peptide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab243096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Involvement in diseaseDefects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
Sequence similaritiesBelongs to the ferritin family.
Contains 1 ferritin-like diiron domain.
- Information by UniProt
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
All lanes : Anti-Ferritin Light Chain antibody [CPT-R10-58-11] (ab243096) at 1/1000 dilution
Lane 1 : NCI-H460 (human lung cancer cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Human brain lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time : Lane 1-4: 26 seconds, Lane 5: 5.5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling Ferritin Light Chain with ab243096 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
50ng CPTAC-14d (1000ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000ng/mL) of ab243096 (50μL) were incubated with CPTAC-14d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.
ab243096 has not yet been referenced specifically in any publications.