Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)

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Overview

  • Product name

    Anti-Ferritin Light Chain antibody [EPR5260]
    See all Ferritin Light Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5260] to Ferritin Light Chain

  • Host species

    Rabbit

  • Specificity

    ab109373 recognizes the light chain of Ferritin only.

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ferritin Light Chain aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, HepG2, and HeLa cell lysates, HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.

    • Involvement in disease

      Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
      Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.

    • Sequence similarities

      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • FRIL_HUMAN antibody
      • FTL antibody
      • LFTD antibody
      • NBIA 3 antibody
      • NBIA3 antibody
      see all

    Images

    • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 20 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)Li W et al., 10(1):e0117435. Fig 5 (A-B). doi:10.1371/journal.pone.0117435. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

      Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).

    • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 20 kDa
      Observed band size: 19 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      Flow Cytometry - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
      Flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Wang K  et al. Correction of Anemia in Chronic Kidney Disease With Angelica sinensis Polysaccharide via Restoring EPO Production and Improving Iron Availability. Front Pharmacol 9:803 (2018). Read more (PubMed: 30108502) »
    • Mertens C  et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS One 11:e0166164 (2016). Flow Cyt ; Human . Read more (PubMed: 27806101) »
    See all 3 Publications for this product

    Publishing research using ab109373? Please let us know so that we can cite the reference in this datasheet.

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