Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)

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Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
  • Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
  • Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5260] to Ferritin Light Chain
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Ferritin Light Chain antibody [EPR5260]
    See all Ferritin Light Chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5260] to Ferritin Light Chain

  • Host species

    Rabbit

  • Specificity

    ab109373 recognizes the light chain of Ferritin only.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, Hap1, HepG2 and HeLa cell lysates. Flow Cyt (intra): HeLa cells. IHC-P: Mouse brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
Application notes
Is unsuitable for ICC or IHC-P.

Target

  • Function

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.

  • Involvement in disease

    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.

  • Sequence similarities

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Ferritin L chain antibody
    • Ferritin L subunit antibody
    • Ferritin light chain antibody
    • Ferritin light polypeptide antibody
    • ferritin light polypeptide like 3 antibody
    • FRIL_HUMAN antibody
    • FTL antibody
    • LFTD antibody
    • NBIA 3 antibody
    • NBIA3 antibody
    see all

Images

  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/2000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : Ferritin knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa



    Lanes 1-2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 20 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)Li W et al., 10(1):e0117435. Fig 5 (A-B). doi:10.1371/journal.pone.0117435. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).

  • Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

    Lane 1 : Fetal liver lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)

    Intracellular flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

     

  • Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
    Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)

References (7)

Publishing research using ab109373? Please let us know so that we can cite the reference in this datasheet.

ab109373 has been referenced in 7 publications.

  • Zuo Y  et al. Sevoflurane anesthesia during pregnancy in mice induces cognitive impairment in the offspring by causing iron deficiency and inhibiting myelinogenesis. Neurochem Int 135:104693 (2020). PubMed: 32035889
  • Xu Y  et al. Astrocyte hepcidin ameliorates neuronal loss through attenuating brain iron deposition and oxidative stress in APP/PS1 mice. Free Radic Biol Med 158:84-95 (2020). PubMed: 32707154
  • Zhang X  et al. Hepcidin overexpression in astrocytes alters brain iron metabolism and protects against amyloid-ß induced brain damage in mice. Cell Death Discov 6:113 (2020). PubMed: 33298837
  • Wang K  et al. Correction of Anemia in Chronic Kidney Disease With Angelica sinensis Polysaccharide via Restoring EPO Production and Improving Iron Availability. Front Pharmacol 9:803 (2018). PubMed: 30108502
  • Khiroya H  et al. IRP2 as a potential modulator of cell proliferation, apoptosis and prognosis in nonsmall cell lung cancer. Eur Respir J 49:N/A (2017). PubMed: 28404645
  • Mertens C  et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS One 11:e0166164 (2016). Flow Cyt ; Human . PubMed: 27806101
  • Li W  et al. Systemic and cerebral iron homeostasis in ferritin knock-out mice. PLoS One 10:e0117435 (2015). WB, IHC-P ; Mouse . PubMed: 25629408

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