Recombinant Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.com/ps/products/109/ab109373/Images/ab109373-389695-anti-ferritin-light-chain-antibody-epr5260-western-blot-human-lysate.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5260] to Ferritin Light Chain
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ferritin Light Chain antibody [EPR5260]
See all Ferritin Light Chain primary antibodies -
Description
Rabbit monoclonal [EPR5260] to Ferritin Light Chain -
Host species
Rabbit -
Specificity
ab109373 recognizes the light chain of Ferritin only. -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, Hap1, HepG2 and HeLa cell lysates. Flow Cyt (intra): HeLa cells. IHC-P: Mouse brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5260 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
Involvement in disease
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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Database links
- Entrez Gene: 2512 Human
- Entrez Gene: 14325 Mouse
- Entrez Gene: 29292 Rat
- Omim: 134790 Human
- SwissProt: P02792 Human
- SwissProt: P29391 Mouse
- SwissProt: P02793 Rat
- Unigene: 433670 Human
see all -
Alternative names
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
Images
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Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Ferritin knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDaLanes 1-2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.com/ps/products/109/ab109373/Images/ab109373-319298-anti-ferritin-light-chain-antibody-epr5260-western-blot.jpg)
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 20 kDaLanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.com/ps/products/109/ab109373/Images/ab109373-269713-anti-ferritin-light-chain-antibody-epr5260-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)Li W et al., 10(1):e0117435. Fig 5 (A-B). doi:10.1371/journal.pone.0117435. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).
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![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.com/ps/products/109/ab109373/Images/ab109373-122150-anti-ferritin-light-chain-antibody-epr5260-western-blot.jpg)
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.com/ps/products/109/ab109373/Images/ab109373-122149-anti-ferritin-light-chain-antibody-epr5260-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)Intracellular flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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![Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.com/ps/products/109/ab109373/Images/ab109373-6-benefits-of-recombinant-antibodies.png)
Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
Datasheets and documents
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Datasheet download
References (10)
ab109373 has been referenced in 10 publications.
- Vara-Pérez M et al. BNIP3 promotes HIF-1a-driven melanoma growth by curbing intracellular iron homeostasis. EMBO J 40:e106214 (2021). PubMed: 33932034
- Wang P et al. Mitochondrial ferritin attenuates cerebral ischaemia/reperfusion injury by inhibiting ferroptosis. Cell Death Dis 12:447 (2021). PubMed: 33953171
- Zuo Y et al. Sevoflurane anesthesia during pregnancy in mice induces cognitive impairment in the offspring by causing iron deficiency and inhibiting myelinogenesis. Neurochem Int 135:104693 (2020). PubMed: 32035889
- Xu Y et al. Astrocyte hepcidin ameliorates neuronal loss through attenuating brain iron deposition and oxidative stress in APP/PS1 mice. Free Radic Biol Med 158:84-95 (2020). PubMed: 32707154
- Zhang X et al. Hepcidin overexpression in astrocytes alters brain iron metabolism and protects against amyloid-ß induced brain damage in mice. Cell Death Discov 6:113 (2020). PubMed: 33298837
- Kong WN et al. The a1-adrenergic receptor is involved in hepcidin upregulation induced by adrenaline and norepinephrine via the STAT3 pathway. J Cell Biochem 119:5517-5527 (2018). PubMed: 29377263
- Wang K et al. Correction of Anemia in Chronic Kidney Disease With Angelica sinensis Polysaccharide via Restoring EPO Production and Improving Iron Availability. Front Pharmacol 9:803 (2018). PubMed: 30108502
- Khiroya H et al. IRP2 as a potential modulator of cell proliferation, apoptosis and prognosis in nonsmall cell lung cancer. Eur Respir J 49:N/A (2017). PubMed: 28404645
- Mertens C et al. Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression. PLoS One 11:e0166164 (2016). Flow Cyt ; Human . PubMed: 27806101
- Li W et al. Systemic and cerebral iron homeostasis in ferritin knock-out mice. PLoS One 10:e0117435 (2015). WB, IHC-P ; Mouse . PubMed: 25629408
