Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)

Overview

  • Product name

    Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free
    See all Ferritin Light Chain primary antibodies
  • Description

    Rabbit monoclonal [EPR5260] to Ferritin Light Chain - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ferritin Light Chain aa 150-250 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab239971 is the carrier-free version of ab109373. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239971 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
    • Involvement in disease

      Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
      Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
    • Sequence similarities

      Belongs to the ferritin family.
      Contains 1 ferritin-like diiron domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • FRIL_HUMAN antibody
      • FTL antibody
      • LFTD antibody
      • NBIA 3 antibody
      • NBIA3 antibody
      see all

    Images

    • All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 20 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

    • Immunohistochemical analysis of wild type and ftl knock out mouse brain tissue labeling Ferritin Light Chain. Positive staining of Ferratin Light Chain is seen in wild type mouse brain (A) and no staining is seen in the ftl knock out brain tissue (B).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

    • Flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109373).

    References

    ab239971 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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