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  1. Link

    ferritin-light-chain-antibody-ftl1386-ab218400.pdf

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Validated using a knockout cell line

Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

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Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

Key features and details

  • Mouse monoclonal [FTL/1386] to Ferritin Light Chain
  • Suitable for: Protein Array, WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2b

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Recombinant Human Ferritin Light Chain protein (ab158473)
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Knockout
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Human FTL knockout HeLa cell line (ab265534)

View more associated products

Overview

  • Product name

    Anti-Ferritin Light Chain antibody [FTL/1386]
    See all Ferritin Light Chain primary antibodies
  • Description

    Mouse monoclonal [FTL/1386] to Ferritin Light Chain
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Ferritin Light Chain aa 38-165. The exact sequence is proprietary.
    Database link: P02792

  • Positive control

    • WB: HAP1, A549, A431, HeLa cell lysates; Human liver, kidney, and testis lysates; IHC-P: Human pancreas and testicular carcinoma tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab218400 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    FTL/1386
  • Isotype

    IgG2b
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Associated products

  • Alternative Versions

    • Anti-Ferritin Light Chain antibody [FTL/1386] - BSA and Azide free (ab218866)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • KO cell lines

    • Human FTL knockout HeLa cell line (ab265533)
    • Human FTL knockout HeLa cell line (ab265534)
  • KO cell lysates

    • Human FTL knockout HeLa cell lysate (ab256926)
    • Human FTL knockout HeLa cell lysate (ab256927)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Recombinant Human Ferritin Light Chain protein (ab158473)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Ferritin Light Chain protein (ab158473)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
  • Involvement in disease

    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.
  • Sequence similarities

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Target information above from: UniProt accession P02792 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2512 Human
    • Omim: 134790 Human
    • SwissProt: P02792 Human
    • Unigene: 433670 Human
    • Unigene: 713706 Human
    • Alternative names

      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • FRIL_HUMAN antibody
      • FTL antibody
      • LFTD antibody
      • NBIA 3 antibody
      • NBIA3 antibody
      see all

    Images

    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : FTL knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 20 kDa
      Observed band size: 20 kDa



      Lanes 1- 2: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) observed at 50 kDa.

       ab218400 was shown to react with Ferritin Light Chain in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265534 (knockout cell lysate ab256927) was used. Wild-type HeLa and FTL knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab218400 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular carcinoma tissue labelling Ferritin Light Chain with ab218400 at 0.5 µg/mL (30 minutes at room temperature). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 2 µg/ml + Human kidney tissue lysate

      Predicted band size: 20 kDa

    • Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      ab218400 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : FTL knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 20 kDa



      Lanes 1-2: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - loading control ab181602 observed at 37 kDa.

       ab218400 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab218400 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate
      Lane 3 : A549 whole cell lysate
      Lane 4 : Hep G2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 20 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab218400 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab218400 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 0.2 µg/ml

      Lanes 1-2 : HeLa cell lysate
      Lane 3 : Human liver lysate
      Lane 4 : Human testis lysate

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab218400? Please let us know so that we can cite the reference in this datasheet.

    ab218400 has been referenced in 2 publications.

    • Terzi EM  et al. Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5. Sci Adv 7:N/A (2021). PubMed: 34039609
    • Chen X  et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). PubMed: 28858244

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