Validated using a knockout cell line

Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

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Overview

  • Product name

    Anti-Ferritin Light Chain antibody [FTL/1386]
    See all Ferritin Light Chain primary antibodies

  • Description

    Mouse monoclonal [FTL/1386] to Ferritin Light Chain

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Ferritin Light Chain aa 38-165. The exact sequence is proprietary.
    Database link: P02792

  • Positive control

    • A431, HeLa cell lysates; Human liver and testis lysates; Human pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 0.1 - 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.

  • Involvement in disease

    Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
    Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels.

  • Sequence similarities

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ferritin L chain antibody
      • Ferritin L subunit antibody
      • Ferritin light chain antibody
      • Ferritin light polypeptide antibody
      • ferritin light polypeptide like 3 antibody
      • FRIL_HUMAN antibody
      • FTL antibody
      • LFTD antibody
      • NBIA 3 antibody
      • NBIA3 antibody
      see all

    Images

    • Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      ab218400 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate
      Lane 3 : A549 whole cell lysate
      Lane 4 : Hep G2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 20 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab218400 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab218400 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 0.2 µg/ml

      Lanes 1-2 : HeLa cell lysate
      Lane 3 : Human liver lysate
      Lane 4 : Human testis lysate

      Predicted band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.

    References

    This product has been referenced in:

    • Chen X  et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). Read more (PubMed: 28858244) »
    See 1 Publication for this product

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