Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-449197-anti-ferritin-light-chain-antibody-ftl1386-western-blot-hela-lysates.jpg "Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)")
Key features and details
- Mouse monoclonal [FTL/1386] to Ferritin Light Chain
- Suitable for: Protein Array, WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-Ferritin Light Chain antibody [FTL/1386]
See all Ferritin Light Chain primary antibodies -
Description
Mouse monoclonal [FTL/1386] to Ferritin Light Chain -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Ferritin Light Chain aa 38-165. The exact sequence is proprietary.
Database link: P02792 -
Positive control
- HAP1, A549, A431, HeLa cell lysates; Human liver and testis lysates; Human pancreas tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab218400 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
FTL/1386 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab218400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa. | |
| IHC-P | Use a concentration of 0.1 - 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
Involvement in disease
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
-
Database links
- Entrez Gene: 2512 Human
- Omim: 134790 Human
- SwissProt: P02792 Human
- Unigene: 433670 Human
- Unigene: 713706 Human
-
Alternative names
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
Images
-
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FTL knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDaLanes 1- 2: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) observed at 50 kDa.
ab218400 was shown to react with Ferritin Light Chain in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265534 (knockout cell lysate ab256927) was used. Wild-type HeLa and FTL knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab218400 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-336435-anti-ferritin-light-chain-antibody-ftl-1386-protein-array.jpg)
Protein Array - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)ab218400 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-389687-anti-ferritin-light-chain-antibody-ftl1386-western-blot-human-lysate.jpg)
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FTL knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDaLanes 1-2: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - loading control ab181602 observed at 37 kDa.
ab218400 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab218400 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-311232-anti-ferritin-light-chain-antibody-ftl-1386-western-blot.jpg)
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : Hep G2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 20 kDaLanes 1 - 4: Merged signal (red and green). Green - ab218400 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab218400 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab218400 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-273740-anti-ferritin-light-chain-antibody-ftl-1386-western-blot.jpg)
Western blot - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)All lanes : Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400) at 0.2 µg/ml
Lanes 1-2 : HeLa cell lysate
Lane 3 : Human liver lysate
Lane 4 : Human testis lysate
Predicted band size: 20 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-273739-anti-ferritin-light-chain-antibody-ftl-1386-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)](https://www.abcam.com/ps/products/218/ab218400/Images/ab218400-273738-anti-ferritin-light-chain-antibody-ftl-1386-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody [FTL/1386] (ab218400)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Ferritin Light Chain with ab218400 at 0.2 μg/ml.
Protocols
Datasheets and documents
References (1)
ab218400 has been referenced in 1 publication.
- Chen X et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). PubMed: 28858244
