Recombinant Anti-FES antibody [EPR3230(2)] (ab108418)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3230(2)] to FES
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FES antibody [EPR3230(2)]
See all FES primary antibodies -
Description
Rabbit monoclonal [EPR3230(2)] to FES -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- TF-1, U937, and Human spleen lysates; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3230(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa). |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/100. |
Target
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Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. - Information by UniProt
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Database links
- Entrez Gene: 2242 Human
- Omim: 190030 Human
- SwissProt: P07332 Human
- Unigene: 7636 Human
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Alternative names
- c-fes/fps protein antibody
- cFES antibody
- Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog antibody
see all
Images
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All lanes : Anti-FES antibody [EPR3230(2)] (ab108418) at 1/1000 dilution
Lane 1 : TF-1 lysate
Lane 2 : U937 lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa -
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FES with purified ab108418 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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ab108418, at a 1/100 dilution, staining FES in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108418 has not yet been referenced specifically in any publications.