Key features and details
- Sheep polyclonal to fetal hemoglobin
- Reacts with: Human
- Isotype: IgG
Product nameAnti-fetal hemoglobin antibody
See all fetal hemoglobin primary antibodies
DescriptionSheep polyclonal to fetal hemoglobin
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Human Hemoglobin F
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was isolated by affinity chromato-graphy using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
RelevanceThe gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab19364 has been referenced in 4 publications.
- Wolk M & Martin JE Fetal haemopoiesis marking low-grade urinary bladder cancer. Br J Cancer 107:477-81 (2012). PubMed: 22735903
- Wolk M & Martin JE Fetal haemoglobin (HbF) as an immunohistochemical tumour marker in bone marrow and spleen. J Clin Pathol 64:645-7 (2011). IHC-P ; Human . PubMed: 21378008
- Yang M et al. Effects of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells. J Ethnopharmacol 127:242-50 (2010). WB . PubMed: 19922785
- Zhang X et al. In vitro maturation of erythroid progenitors from human umbilical cord blood and patterns of globin gene expression: serum from different developmental stage plays important roles in liquid culture. Biochem Biophys Res Commun 336:42-8 (2005). PubMed: 16125140