Anti-fetal hemoglobin antibody (ab19364)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-fetal hemoglobin antibody
See all fetal hemoglobin primary antibodies -
DescriptionSheep polyclonal to fetal hemoglobin
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Host speciesSheep
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SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
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Tested applicationsSuitable for: ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Human Hemoglobin F
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody was isolated by affinity chromato-graphy using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab19364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent dilution. | |
| IHC-P | 1/20. Antigen retrieval is not essential but may optimise staining. PubMed: 21378008 |
Target
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RelevanceThe gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
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Cellular localizationCytoplasmic
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Database links
- Entrez Gene: 3047 Human
- Entrez Gene: 3048 Human
- Omim: 142200 Human
- Omim: 142250 Human
- SwissProt: P69891 Human
- SwissProt: P69892 Human
- Unigene: 302145 Human
- Unigene: 702189 Human
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Alternative names
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
see all
Protocols
References
This product has been referenced in:
- Wolk M & Martin JE Fetal haemopoiesis marking low-grade urinary bladder cancer. Br J Cancer 107:477-81 (2012). Read more (PubMed: 22735903) »
- Wolk M & Martin JE Fetal haemoglobin (HbF) as an immunohistochemical tumour marker in bone marrow and spleen. J Clin Pathol 64:645-7 (2011). IHC-P ; Human . Read more (PubMed: 21378008) »
