Recombinant
RabMAb

Recombinant Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)

Overview

  • Product name

    Anti-fetal hemoglobin antibody [EPR9708(B)]
    See all fetal hemoglobin primary antibodies
  • Description

    Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin
  • Host species

    Rabbit
  • Specificity

    ab137096 recognises Hemoglobin gamma-1/2 chain.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human fetal hemoglobin. The exact sequence is proprietary.

  • Positive control

    • WB: Human red blood cell and K562 cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9708(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance

      The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
    • Cellular localization

      Cytoplasmic
    • Database links

    • Alternative names

      • A gamma globin antibody
      • G gamma globin antibody
      • Gamma 1 globin antibody
      • Gamma 2 globin antibody
      • Gamma A hemoglobin antibody
      • Gamma globin antibody
      • Hb F Agamma antibody
      • Hb F Ggamma antibody
      • hbfagamma antibody
      • hbfggamma antibody
      • HBG 1 antibody
      • HBG 2 antibody
      • HBG1 antibody
      • HBG2 antibody
      • HBGA antibody
      • HBGR antibody
      • Hemoglobin gamma 1 chain antibody
      • Hemoglobin gamma 2 chain antibody
      • Hemoglobin gamma A antibody
      • Hemoglobin gamma A chain antibody
      • Hemoglobin gamma G antibody
      • Hemoglobin gamma G chain antibody
      • Hemoglobin gamma regulator of antibody
      • Hemoglobin subunit gamma 1 antibody
      • Hemoglobin subunit gamma 2 antibody
      • HSGGL 1 antibody
      • HSGGL1 antibody
      • Methemoglobin antibody
      • PRO 2979 antibody
      • PRO2979 antibody
      see all

    Images

    • Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 16 kDa
      Observed band size: 13 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Unpurified)

      Lane 1 : Human red blood cell
      Lane 2 : K562 (human lymphoblast from chronic myelogenous leukemia) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

      Predicted band size: 16 kDa



      Blocking and Diluting Buffer:5% NFDM/TBST

       

      Exposure time:3 min

    References

    This product has been referenced in:

    • Sun KT  et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). WB . Read more (PubMed: 28303002) »
    • Li J  et al. SOX6 Downregulation Induces?-Globin in Humanß-Thalassemia Major Erythroid Cells. Biomed Res Int 2017:9496058 (2017). Read more (PubMed: 29333458) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer


    For the IHC images, the age of the spleen donor is 44, and the lung donor's age was 66. For the WB image, we used adult RBC's (age unknown).

    I'm still checking with the lab regarding the fetal protein being expressed in adult tissues/cells. I'll let you know what I find out.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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