Product nameAnti-fetal hemoglobin antibody [EPR9709]
See all fetal hemoglobin primary antibodies
DescriptionRabbit monoclonal [EPR9709] to fetal hemoglobin
Specificityab156584 recognises Hemoglobin gamma-1/2 chain.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human fetal hemoglobin. The exact sequence is proprietary.
- Red blood cells and K562 lysates, K562 cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 15 kDa.|
RelevanceThe gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
All lanes : Anti-fetal hemoglobin antibody [EPR9709] (ab156584) at 1/2000 dilution
Lane 1 : Human red blood cell
Lane 2 : K562(human lymphoblast from chronic myelogenous leukemia) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 15 kDa
Blocking and Diluting buffer:5% NFDM/TBST
Exposure time:3 min
This product has been referenced in:
- Pule GD et al. Hydroxyurea down-regulates BCL11A, KLF-1 and MYB through miRNA-mediated actions to induce ?-globin expression: implications for new therapeutic approaches of sickle cell disease. Clin Transl Med 5:15 (2016). WB ; Human . Read more (PubMed: 27056246) »