Overview

  • Product name

    Anti-fetal hemoglobin antibody (FITC)
    See all fetal hemoglobin primary antibodies
  • Description

    Sheep polyclonal to fetal hemoglobin (FITC)
  • Host species

    Sheep
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
  • Tested applications

    Suitable for: ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Human Hemoglobin F

  • General notes

    F/P ratio is 3 to 7.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200 - 1/2000.

Target

  • Relevance

    The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • A gamma globin antibody
    • G gamma globin antibody
    • Gamma 1 globin antibody
    • Gamma 2 globin antibody
    • Gamma A hemoglobin antibody
    • Gamma globin antibody
    • Hb F Agamma antibody
    • Hb F Ggamma antibody
    • hbfagamma antibody
    • hbfggamma antibody
    • HBG 1 antibody
    • HBG 2 antibody
    • HBG1 antibody
    • HBG2 antibody
    • HBGA antibody
    • HBGR antibody
    • Hemoglobin gamma 1 chain antibody
    • Hemoglobin gamma 2 chain antibody
    • Hemoglobin gamma A antibody
    • Hemoglobin gamma A chain antibody
    • Hemoglobin gamma G antibody
    • Hemoglobin gamma G chain antibody
    • Hemoglobin gamma regulator of antibody
    • Hemoglobin subunit gamma 1 antibody
    • Hemoglobin subunit gamma 2 antibody
    • HSGGL 1 antibody
    • HSGGL1 antibody
    • Methemoglobin antibody
    • PRO 2979 antibody
    • PRO2979 antibody
    see all

References

This product has been referenced in:

  • Starlard-Davenport A  et al. MIR29B mediates epigenetic mechanisms of HBG gene activation. Br J Haematol N/A:N/A (2019). Read more (PubMed: 30891745) »
  • Oseghale AR  et al. Conjugate prodrug AN-233 induces fetal hemoglobin expression in sickle erythroid progenitors and ß-YAC transgenic mice. Blood Cells Mol Dis 79:102345 (2019). Read more (PubMed: 31351219) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Question
Answer



This antibody was generated against a whole hemoglobin prep’, and does not allow discrimination between the tetramer and subunits. As a true polyclonal antibody, there may be one or more members of the clonal “pool” which bind exclusively to either the tetramer or the subunit, but it is not our intention for the material to be used to discriminate among these different forms of the molecule.

Read More

Question
Answer

DISCOUNT CODE: XXXXXXXX
Expiration date: 29-05-2013
Value: £235

I am very pleased to hear you would like to accept our offer and test ab19365 in Flow Cytometry. This code will give you £235 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

PS: In the following publication researcher have used similar FITC conjugated antibody in Flow Cytometry - the protocol may be helpful.

http://ajcp.ascpjournals.org/content/117/6/857.full.pdf

http://discovery.ucl.ac.uk/108952/

Read More

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