Key features and details
- Goat polyclonal to Fetuin
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Fetuin antibody
DescriptionGoat polyclonal to Fetuin
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant fragment (alpha 2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein (Human).
- WB: Human serum or purified Fetuin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesAb34505 was purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Our Abpromise guarantee covers the use of ab34505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10000 - 1/20000. Detects a band of approximately 58 kDa.
Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPromotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
Tissue specificitySynthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Sequence similaritiesBelongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains.
modificationsPhosphorylation sites are present in the extracelllular medium.
O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).
- Information by UniProt
- 16G2 antibody
- A2HS antibody
- AHS antibody
All lanes : Anti-Fetuin antibody (ab34505) at 1/10000 dilution
Lane 1 : 250 ng of purified
Lane 2 : 5µl of human serum at 1/50 dilution
Observed band size: 58 kDa why is the actual band size different from the predicted?
A doublet band or slightly lower molecular weight band (see lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.
ab34505 has been referenced in 1 publication.
- Kutikhin AG et al. Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions. Sci Rep 6:27255 (2016). WB . PubMed: 27251104