Overview

  • Product name

  • Description

    Goat polyclonal to Fetuin
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment (alpha 2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein (Human).

  • Positive control

    • WB: Human serum or purified Fetuin.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Ab34505 was purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/40,000 - 1/200,000.
    WB: 1/10000 - 1/20000. Detects a band of approximately 58 kDa.

    Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
    • Tissue specificity

      Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
    • Sequence similarities

      Belongs to the fetuin family.
      Contains 2 cystatin fetuin-A-type domains.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
      O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • 16G2 antibody
      • A2HS antibody
      • AHS antibody
      • Ahsg antibody
      • Alpha 2 HS glycoprotein antibody
      • Alpha 2 Z globulin antibody
      • Alpha 2HS glycoprotein antibody
      • Alpha-2-HS-glycoprotein chain B antibody
      • Alpha-2-Z-globulin antibody
      • Ba alpha 2 glycoprotein antibody
      • Ba-alpha-2-glycoprotein antibody
      • FETUA antibody
      • FETUA_HUMAN antibody
      • FETUB antibody
      • Fetuin A antibody
      • Fetuin B antibody
      • Fetuin like protein antibody
      • Fetuin-A antibody
      • FetuinA antibody
      • FetuinB antibody
      • Gugu antibody
      • HSGA antibody
      • IRL 685 antibody
      • IRL685 antibody
      • PRO2743 antibody
      see all

    Images

    • All lanes : Anti-Fetuin antibody (ab34505) at 1/10000 dilution

      Lane 1 : 250 ng of purified
      human Fetuin
      Lane 2 : 5µl of human serum at 1/50 dilution

      Observed band size: 58 kDa
      why is the actual band size different from the predicted?



      A doublet band or slightly lower molecular weight band (see lane 1) may be visible by western blot due to proteolytic processing, variable glycosylation and/or phosphorylation. Proteolytic processing may include the removal of a 40 amino acid residue bridging peptide from the A and B chains of fetuin in vivo.

    References

    This product has been referenced in:

    • Kutikhin AG  et al. Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions. Sci Rep 6:27255 (2016). WB . Read more (PubMed: 27251104) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab34505.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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