Recombinant
RabMAb

Recombinant Anti-Fetuin B antibody [EPR16236] (ab191569)

Overview

  • Product name

    Anti-Fetuin B antibody [EPR16236]
    See all Fetuin B primary antibodies
  • Description

    Rabbit monoclonal [EPR16236] to Fetuin B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human Fetuin B aa 200-350. The exact sequence is proprietary.
    Database link: Q9UGM5

  • Positive control

    • Human plasma and serum; Human and Rat liver tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16236
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical staining of Rat liver tissue labeling Feutin B using ab191569 at 1/600 dilution (1μg/ml). Negative control (inset) using PBS instead of ab191569. Secondary antibody is a ready to use HRP Polymer for Rabbit/Mouse IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical staining of Human liver tissue labeling Feutin B using ab191569 at 1/600 dilution (1μg/ml). Negative control (inset) using PBS instead of ab191569. Secondary antibody is a ready to use HRP Polymer for Rabbit/Mouse IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Fetuin B antibody [EPR16236] (ab191569) at 1/10000 dilution + Human serum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa



    5% NFDM/TBST blocking buffer

  • Anti-Fetuin B antibody [EPR16236] (ab191569) at 1/1000 dilution + Human plasma at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

References

This product has been referenced in:

  • Zhou W  et al. Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice. Am J Transl Res 11:1498-1509 (2019). Read more (PubMed: 30972177) »
  • Joshi S  et al. Transcriptional study of hyperoxaluria and calcium oxalate nephrolithiasis in male rats: Inflammatory changes are mainly associated with crystal deposition. PLoS One 12:e0185009 (2017). Read more (PubMed: 29091707) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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