RecombinantRabMAb

Recombinant Anti-Fetuin B antibody [EPR16236] (ab191569)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin B antibody [EPR16236] (ab191569)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin B antibody [EPR16236] (ab191569)
  • Western blot - Anti-Fetuin B antibody [EPR16236] (ab191569)
  • Western blot - Anti-Fetuin B antibody [EPR16236] (ab191569)
  • Anti-Fetuin B antibody [EPR16236] (ab191569)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16236] to Fetuin B
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Fetuin B antibody [EPR16236]
    See all Fetuin B primary antibodies

  • Description

    Rabbit monoclonal [EPR16236] to Fetuin B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment within Human Fetuin B aa 200-350. The exact sequence is proprietary.
    Database link: Q9UGM5

  • Positive control

    • Human plasma and serum; Human and Rat liver tissues

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16236

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin B antibody [EPR16236] (ab191569)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin B antibody [EPR16236] (ab191569)

    Immunohistochemical staining of Rat liver tissue labeling Feutin B using ab191569 at 1/600 dilution (1μg/ml). Negative control (inset) using PBS instead of ab191569. Secondary antibody is a ready to use HRP Polymer for Rabbit/Mouse IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin B antibody [EPR16236] (ab191569)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fetuin B antibody [EPR16236] (ab191569)

    Immunohistochemical staining of Human liver tissue labeling Feutin B using ab191569 at 1/600 dilution (1μg/ml). Negative control (inset) using PBS instead of ab191569. Secondary antibody is a ready to use HRP Polymer for Rabbit/Mouse IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Fetuin B antibody [EPR16236] (ab191569)
    Western blot - Anti-Fetuin B antibody [EPR16236] (ab191569)
    Anti-Fetuin B antibody [EPR16236] (ab191569) at 1/10000 dilution + Human serum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa



    5% NFDM/TBST blocking buffer

  • Western blot - Anti-Fetuin B antibody [EPR16236] (ab191569)
    Western blot - Anti-Fetuin B antibody [EPR16236] (ab191569)
    Anti-Fetuin B antibody [EPR16236] (ab191569) at 1/1000 dilution + Human plasma at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 42 kDa

  • Anti-Fetuin B antibody [EPR16236] (ab191569)
    Anti-Fetuin B antibody [EPR16236] (ab191569)

References (2)

Publishing research using ab191569? Please let us know so that we can cite the reference in this datasheet.

ab191569 has been referenced in 2 publications.

  • Zhou W  et al. Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice. Am J Transl Res 11:1498-1509 (2019). PubMed: 30972177
  • Joshi S  et al. Transcriptional study of hyperoxaluria and calcium oxalate nephrolithiasis in male rats: Inflammatory changes are mainly associated with crystal deposition. PLoS One 12:e0185009 (2017). PubMed: 29091707

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