Recombinant Anti-Fetuin B antibody [EPR16236] (ab191569)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16236] to Fetuin B
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fetuin B antibody [EPR16236]
See all Fetuin B primary antibodies -
Description
Rabbit monoclonal [EPR16236] to Fetuin B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma and serum; Human and Rat liver tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16236 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Liver and testis. -
Sequence similarities
Belongs to the fetuin family.
Contains 2 cystatin fetuin-B-type domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 26998 Human
- Entrez Gene: 83928 Rat
- Omim: 605954 Human
- SwissProt: Q9UGM5 Human
- SwissProt: Q9QX79 Rat
- Unigene: 81073 Human
- Unigene: 2464 Rat
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Alternative names
- 16G2 antibody
- 2310011O17Rik antibody
- AI255764 antibody
see all
Images
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Immunohistochemical staining of Rat liver tissue labeling Feutin B using ab191569 at 1/600 dilution (1μg/ml). Negative control (inset) using PBS instead of ab191569. Secondary antibody is a ready to use HRP Polymer for Rabbit/Mouse IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical staining of Human liver tissue labeling Feutin B using ab191569 at 1/600 dilution (1μg/ml). Negative control (inset) using PBS instead of ab191569. Secondary antibody is a ready to use HRP Polymer for Rabbit/Mouse IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Fetuin B antibody [EPR16236] (ab191569) at 1/10000 dilution + Human serum at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa5% NFDM/TBST blocking buffer
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Anti-Fetuin B antibody [EPR16236] (ab191569) at 1/1000 dilution + Human plasma at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab191569 has been referenced in 2 publications.
- Zhou W et al. Fetuin B aggravates liver X receptor-mediated hepatic steatosis through AMPK in HepG2 cells and mice. Am J Transl Res 11:1498-1509 (2019). PubMed: 30972177
- Joshi S et al. Transcriptional study of hyperoxaluria and calcium oxalate nephrolithiasis in male rats: Inflammatory changes are mainly associated with crystal deposition. PLoS One 12:e0185009 (2017). PubMed: 29091707