Overview

  • Product name

  • Sample type

    Tissue sections
  • Product overview

    Feulgen Stain Kit ab245878 is a histochemical reagent kit for quantifying the nuclear DNA content in cells. The kit can be used on a variety of specimen types from cytocentrifuge preparations to formalin fixed paraffin embedded tissue sections. A unique formulation eliminates dye precipitation and results in a cleaner stain than conventional Feulgen reagent-based kits. This stain kit is optimized for DNA Ploidy specimens that will be stained then scanned and viewed on image analysis systems. This kit is designed for cytological specimens prepared from cytospins, smears, cell imprints, disaggregated tissue, or whole tissue.


    The amount of stain color developed is directly proportional to the amount of DNA present in the stained cells.


    Staining Interpretation:


    Nuclei: Blue.


    Nucleoli: Light spaces within the nuclei with dark edges.


    Cytoplasm: Transparent with no staining.


    Note: Strongly acidic mucous may occasionally stain blue/green.


    Control Tissue:


    Cytological specimens prepared from whole tissue, disaggregated tissue, cell imprints, smears, or cytospins.


    Avoid Bouin’s stain as it hydrolyses DNA.


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 kit
    Blue Feulgen Decolorizer 10 vials
    Blue Feulgen Rinse Reagent 10 vials
    Blue Fuelgen Stain 2 x 500ml

Protocols

References

ab245878 has not yet been referenced specifically in any publications.

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